过氧化氢酶发酵生产条件优化及在染整清洁生产中的应用研究  

Fermentation optimization and textile pretreatment application of catalase produced by Thermoascus aurantiacus WSH 03-01

作　　者：曹翔宇[1] 华兆哲[1] 燕国梁[1] 堵国成[1] 陈坚[1] 

机构地区：[1]江南大学生物工程学院环境生物技术研究室,江苏无锡214036

出　　处：《工业微生物》2006年第4期7-12,共6页Industrial Microbiology

基　　金：国家高技术研究发展计划(863)项目资助(No.2003AA322050);江苏省创新人才(学术)项目资助(No.BK2004401)

摘　　要：在以嗜热子囊菌生产过氧化氢酶的摇瓶发酵研究中,获得了适合工业化生产的碳源,即以26g/L的糊化玉米淀粉和1%(v/v)乙醇为混合碳源,过氧化氢酶的酶活达到了1996 u/mL,比优化前提高了25%。在此基础上,重点研究了发酵罐上的主要影响因素溶解氧对发酵的影响,通过分段控制搅拌转速,在52h将搅拌转速从200r/min提高到350r/min,过氧化氢酶最高酶活达到4505 u/mL,与不使用控制溶氧水平策略相比,酶活力提高了2.4倍。此外,还考察了该酶去除过氧化氢的效率,并在实际纺织生产中进行了应用实验,取得了良好的效果。The effects of different carbon sources on industrialized production of catalase （CAT） by Thermoascus aurantiacus WSH 03-01 were investigated in shake-flask culture. The highest activity of CAT （1996 u/mL） was obtained in complex carbon source medium containing 26g/L cornstarch and 1% ethanol （v/v）, which was 25 % higher than that of original medium. It was also found that dissolved oxygen concentration was an important factor affecting CAT synthesis. Therefore, a two-stage agitation speed control strategy was developed in 7 L fermentor, in which the agitation was controlled at 200 r/rain in the first 52 h, and then switched to 350 r/min. By applying this strategy, the CAT activity was increased by 240 % to 4505 u/mL compared with the control which the agitation speed was kept at 200 r/min In addition, the industrialized application of catalase in the textile pretreatment was also carried out.

关 键 词：过氧化氢酶 发酵条件优化 嗜热子囊菌 纺织清洁生产 

分 类 号：TQ925[轻工技术与工程—发酵工程]