头孢菌素C发酵过程代谢特性  被引量:1

Metabolic Properties of Cephalosporin C Fermentation

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作  者:冀志霞[1] 储炬[1] 庄英萍[1] 张嗣良[1] 罗家立 白骅 

机构地区:[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心,上海200237 [2]浙江海正药业股份有限公司,台州318000

出  处:《华东理工大学学报(自然科学版)》2006年第11期1275-1279,1309,共6页Journal of East China University of Science and Technology

基  金:上海市科委重点科技攻关项目(034319220)

摘  要:考察了头孢菌素C的发酵过程特征,对发酵过程的重要生理参数DO、CER、OUR、RQ等进行了在线检测和相关分析,并分析了比生长速率和比产物生成速率的变化规律。在发酵过程中0UR、CER最高分别达到了90.2 mol/(m^3·h)和69.8 mol/(m^3·h),表明头孢菌素C发酵代谢强度很大,对氧的需求量也非常大。比产物生成速率在发酵60~100h最高达到2.15U/(h·g)(细胞干重)。同时分析了头孢菌素C发酵过程中甲硫氨酸代谢规律,表明维持培养基中一定浓度的甲硫氨酸对头孢菌素C的合成有促进作用。通过对三级发酵和四级发酵的比较,表明在现有的设备和工艺条件下,三级发酵生产头孢菌素C要优于四级发酵,同时通过控制发酵前期菌丝浓度可获得最大产素水平。Fermentation process of cephalosporin C was studied. The DO(dissolved oxygen) tension, CER(carbon dioxide evolution rate), OUR (oxygen uptake rate) and RQ(respiratory quotient) were monitored on line and the specific growth rate, specific production rate were analyzed. The highest CER and OUR are 90. 2 mol/(m^3· h) and 69.8 mol/(m^3· h) respectively. It reveals that cephalosporin C biosynthesis is a high oxygen demanding process. The specific production rate reaches its highest value at 60-100 h. At the same time, methionine was analyzed off line. For cephalosporin C biosynthesis it is essential to keep methionine concentration at certain level in the broth. By controlling the biomass at the earlier stage of fermentation under the existing equipments and air compression capacity, the fermentation with twice precultures is found to be superior to that with thrice precultures in terms of productivity.

关 键 词:头孢菌素C 发酵 代谢特性 甲硫氨酸 

分 类 号:TQ920.6[轻工技术与工程—发酵工程]

 

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