转聚磷激酶基因的大肠杆菌去除水体中的磷  

Soluble phosphorus removed by the Escherichia coli transformed with polyphosphate kinase gene.

作　　者：王勤[1] 赵庆顺[2] 肖琳[1] 蒋丽娟[1] 杨柳燕[1] 尹大强[1] 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210093 [2]南京大学模式动物研究所医药生物技术国家重点实验室,江苏南京210061

出　　处：《中国环境科学》2006年第6期742-745,共4页China Environmental Science

基　　金：国家"973"项目(2002CB412307);国家重大水环境专项(2002AA601011);国家自然科学基金资助项目(40371102)

摘　　要：为探索有效的生物除磷方法,构建了聚磷激酶基因(ppk)的表达载体pET28a(+),并转化大肠杆菌(Escherichiacoli)BL21,获得了可过表达ppk基因的工程菌BL-PPK.RT-PCR结果表明,ppk基因在转化的大肠杆菌中得到了高效表达.聚磷试验结果表明,培养10h后,转化了ppk基因的大肠杆菌细胞内聚磷含量比对照菌株高20倍,而培养液中可溶性磷浓度为对照菌株的约1/9.For exploring an effective technique of phosphorus bioremoval, the express carrier pET28a（＋） of polyphosphate kinase （ppk） gene was constructed and transformed as Escherichia coli BL21, and engineering bacteria BL-PPK capable to express ppk gene was obtained. The ppk gene in transformed Escherichia coli obtained highly effective expression. Experiment results of polyphosphate showed that the content of polyphosphate in the Escherichia coli cell transformed to ppk gene was 20 times higher than that in control bacteria after 10 h culture; while in culture liquor, the soluble phosphorus concentration was about 1/9 of the control bacteria.

关 键 词：聚磷激酶(PPK) 聚磷 转基因 大肠杆菌 

分 类 号：X172[环境科学与工程—环境科学]