牛血清的细菌内毒素检测  

Endotoxin Inspection of Bovine Serum

作　　者：李华[1] 宋晓艳[1] 柯华昕[1] 谢忠平[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所,云南昆明650118

出　　处：《中国药业》2007年第1期13-14,共2页China Pharmaceuticals

摘　　要：目的考察牛血清细菌内毒素检查法的可行性。方法采用不同厂家生产的不同批号、不同灵敏度的鲎试剂进行干扰试验,按2005年版《中华人民共和国药典(三部)》附录收载的细菌内毒素凝胶限量检查法进行试验。结果选用灵敏度为0.125EU/mL的鲎试剂时,牛血清稀释80倍后不会对细菌内毒素检查产生干扰。结论用鲎试剂检查牛血清细菌内毒素的方法是可行的。Objective To establish the inspection method of endotoxin in bovine serum. Methods The experiment was implemented according to the endotoxin inspection in China Pharmacopoeia （Ⅲ, 2005 ED）. To study the interferential test of bovine serum using tachypleus ame- bocyte lysate （TAL） with different factories, different batch numbers and different sensitivities. Results The bovine serum did not interfere with the agglutinative reaction of TAL. The bovine serum was assayed by TAL labeled the sensitivity of 0. 125 EU/mL for endotoxin. Conclusion The endotoxin inspection method can be used for bovine serum.

关 键 词：牛血清 细菌内毒素 鲎试剂 干扰试验 

分 类 号：R927.1[医药卫生—药学] R977.8