TNF-α基因单核苷酸多态性与HBV感染结局  

Relationship between polymorphisms of TNF-α gene promoter region and HBV infection outcome

作　　者：勾春燕[1] 李洪权[2] 李卓[1] 刘英[2] 李俊红[1] 曾宪嘉[2] 高冀荣[1] 潘利[2] 郭新会[1] 单晶[1] 李辉[2] 

机构地区：[1]首都医科大学附属北京佑安医院,北京100069 [2]中国医学科学院基础医学研究所流行病学教研室

出　　处：《中国公共卫生》2007年第1期87-88,共2页Chinese Journal of Public Health

基　　金：北京市科委重大项目(H020920020590)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)基因启动子区-238G/A-、857T/C-、863C/A与乙型肝炎病毒(HBV)感染结局之间的关系。方法采用病例-对照研究方法,募集244例HBV自限性感染者、212例HBsAg携带者和391例慢性乙型肝炎患者作为研究对象,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对(TNF-α)基因启动子区-238G/A、-857C/T、-863C/A基因型进行测定。结果自限性感染者携带-238 A等位基因的频率显著低于HBsAg携带者(P=0.04)和慢性乙肝患者(P=0.047);慢性乙肝患者携带TNF-α-857C等位基因的频率显著高于HBsAg携带者(P=0.0008)和自限性感染者(P=0.03);慢性乙肝者TNF-α-863A等位基因频率显著高于HBsAg携带者(P=0.02)。应用多元Logistic回归分析,控制年龄、性别等混杂因素后,慢性乙肝患者与HBV自限性感染者比较,TNF-α-857CC和TNF-α-238 GA与慢性乙肝显著关联(OR=1.53,P=0.044;OR=2.11,P=0.045);慢性乙肝患者与HBsAg携带者比较,TNF-α-857CC与慢性乙肝显著关联(OR=1.92,P=0.004);HBsAg携带者与HBV自限性感染者比较,TNF-α-238GA与HBsAg携带者显著关联(OR=2.34,P=0.020)。结论TNF-α基因启动子区多态性可能是影响HBV感染结局的重要危险因素。Objective To determine whenther - 238G/A, - 857T/C and - 863C/A polymorphisms of tumor necrosis factor- alphya （TNF - α） gene promoter were associated with outcomes of hepatitis B virus （HBV） infection. Methods A total of 244 HBV self - limited infected subjects, 212 asymptomatic HBsAg carriers （ HBsAg carriers） and 391 chronic hepatitis B （HB） patients were recruited to conduct a case - control study. TNF - α - 238G/A, - 857C/T and - 863C/A gene promoter polymorphisms were examined by polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP）. Results The frequency of - 238 allele A in self - limited individuals was 2.6 %, significantly lower than 5.0 % in chronic HB patients and 5.3 % in HBsAg carriers （ P = 0.04 and P = 0. 047）, respectively. The frequency of TNF - α - 857 allele C in chronic HB patients was 87.5 %, significantly higher than 80.5 0,6 in HBsAg carriers and 82.8 % in self - limited individuals （P = 0. 0008 and P = 0.03）, respectively. The frequency of TNF-α- 863 allele A in chronic HB group was 77.5 %, significantly higher than 71.50,6 in HBsAg carriers （ P = 0.02）. Multiple logistic regression analyses indicated an increased risk of chronic HB associated with TNF - α - 238GA and - 857CC after gender and age adjusting （ OR = 1.53, P = 0. 044; OR = 2.11, P = 0. 045）, and HBsAg carriers with - 857CC significantly increasing risk of chronic HB associated with TNF - α- 238GA and - 857CC after gender and age adjusting （ OR = 1.53, P = 0. 044 ; OR = 2.11, P = 0. 045 ）, an HB- sag carriers with - 857CC significantly increasing risk of chronic HB（ OR = 1.92, P = 0. 004）, and - 238GA associated with and increasing risk of HBsAg carriers （ OR = 2.34, P = 0. 020）. Conclusion TNF - α promoter polymorphism is probably an important risk factor of the influence of outcome of HBV infection.

关 键 词：乙型肝炎 TNF-Α基因 单核苷酸多态性(SNPs) 单体型 

分 类 号：R745[医药卫生—神经病学与精神病学]