富硒酵母菌株的选育  

Breeding of Selenium-enriched Yeast Strains

作　　者：朱昌雄[1] 马爱民[1] 刘代武[2] 姚鹃[2] 

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070 [2]安琪酵母股份有限公司,湖北宜昌443003

出　　处：《酿酒科技》2007年第1期46-49,共4页Liquor-Making Science & Technology

摘　　要：以酿酒酵母Saccharomyces cerevisiaeL-11为出发菌株,通过DES多次诱变,Na2SeO3和乙硫氨酸抗性平板选择,得到一株高硒和高蛋氨酸含量的菌株L-11-7-2,其蛋氨酸含量达到1.37%,是L-11的169%。对L-11-7-2进行富硒培养,细胞内总硒和硒蛋氨酸分别为2452μg/g和1650μg/g,分别为出发株的280.2%和369.1%。A Selenium-enriched yeast strain L-11-7-2 was obtained from Saccharomyces cerevisiae L-11 as starting strains by DES mutagenesis and screening on plate containing Na2SeO3 and ethionine. The methionine content of the strain reached 1.37 %, which was 1.69 times higher than that of the parent strain L-11. After Se-enriched culture, the total Se and Selenomethionine content of the L-11-7-2 were 2452 μg/g and 1650 μg/g respectively, 2.802 and 3.691 times higher than that of the parent strain.

关 键 词：微生物 富硒酵母 选育 硒代蛋氨酸 

分 类 号：Q933[生物学—微生物学]