LMP2/LMP7基因编码区多态性及单倍体型与胃癌易感无相关性  

No association between LMP2/LMP7 gene polymorphisms or haplotypes and gastric cancer

作　　者：曹邦伟[1] 戴悦[2] 盖郁慧[2] 柴庆波[2] 汪欣[3] 

机构地区：[1]北京大学第一医院抗感染病房北京大学临床药理研究所,北京市100034 [2]北京大学第一医院输血科,北京市100034 [3]北京大学第一医院普外科,北京市100034

出　　处：《世界华人消化杂志》2006年第36期3471-3476,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨中国汉族人群中肿瘤抗原递呈的限速基因LMP2/LMP7单核苷酸多态性与胃癌的遗传易感性关联.方法:提取145例胃癌患者及152例健康献血员外周血基因组DNA,用PCR-RFLP方法检测两组人群中LMP2-codon60/LMP7-codon145两个多态性位点的基因型;并采用PHASE1.0软件构建这两个多态性位点的个体单倍体型,以非条件Logistic回归校正混杂因素,并进行多态性与胃癌患者风险关联的统计学分析.结果:PCR-RFLP检测结果显示:LMP2-codon60与LMP7-codon145处的多态性在中国人群中普遍存在,LMP2/LMP7多态性位点的各自的等位基因频率分布符合Hardy-Weinberg平衡定律;最后的统计分析表明:在145例胃癌患者与152例健康对照人群中LMP2/LMP7两个多态性位点的基因型频率、以及所构建的4个单倍体型频率均没有统计学差异(P值均大于0.05).结论:在中国汉族人群中,LMP2/LMP7基因编码区两个位点的多态性以及单倍体型与胃癌易感无相关性.AIM： To elucidate whether LMP2/LMP7 polymorphisms or haplotypes were associated with the occurrence of gastric cancer in Han Chinese. METHODS： The genome DNA was extracted from the peripheral blood of 145 gastric cancer patients and 152 cancer-free controls, and the LMP2-codon60/LMP7-codon145 genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR- RFLP）. The software PHASE1.0 was used to construct the haplotypes of each individual. The unconditional Logistic regression model was applied to analyze the statistical differences of genotypes or haplotypes between the two groups. RESULTS： PCR-RFLP showed that the polymorphisms were found at both codon60 and codon145 loci of LMP2/LMP7 gene in 297 Chinese individuals, and chi-square test demonstrate that the distributions of LMP2/LMP7 genotypes were in Hardy-Weinberg equilibrium. After statistical analysis, we found the frequencies of LMP2/LMP7 gene polymorphisms and the four haplotypes were not significantly different between gastric cancer patients and cancer-flee controls （all P 〉 0.05）. CONCLUSION： The polymorphisms and haplotypes of LMP2-codon60/LMP7-codon145 gene may not contribute to the genetic susceptibility of gastric cancer in the Han nationality of China.

关 键 词：低分子量蛋白酶体 胃癌 单倍体型 易感 

分 类 号：R735.2[医药卫生—肿瘤]