survivin反义寡核苷酸诱导白血病细胞K562凋亡的作用  

Effect of antisense oligodeoxynucleotide targeting survivin on the apoptosis of leukemic cell K562

作　　者：阳洁[1] 姜浩[1] 陈艳华[1] 

机构地区：[1]南华大学第一附属医院,湖南省衡阳市421001

出　　处：《实用诊断与治疗杂志》2007年第1期15-16,19,I0001,共4页Journal of Practical Diagnosis and Therapy

摘　　要：目的:观察surv iv in反义寡核苷酸对K 562细胞凋亡的作用。方法:以脂质体转染surv iv in反义寡核苷酸;M TT检测surv iv in反义寡核苷酸对K 562细胞的生长抑制;流式细胞术、荧光分光光度计分别检测细胞凋亡和半胱氨蛋白水解酶3的活性;荧光分光显微镜观察凋亡形态。结果:surv iv in反义寡核苷酸对K 562细胞的生长抑制呈浓度-时间依赖性;400 nm o l/L的A SODN作用48 h后,与对照组相比,K 562细胞的凋亡率增加(18.60±3.46)%vs(1.35±0.31)%,半胱氨蛋白水解酶3的活性增加77.99%。细胞表现为细胞皱缩、核质浓缩、核碎裂、细胞起泡以及凋亡小体形成等凋亡特征的形态学改变。结论:surv iv in反义寡核苷酸能抑制K 562细胞的生长,诱导凋亡。Objective To observe the effect of induction of apoptosis by survivin antisense oligodeoxynucleotide in leukemic cell K562. Methods Survivin antisense oligodeoxynucleotide was transfected to K562 cells with liposome. Cytotoxicity of antisense oligodeoxynucleotide was detected with colorimetric MTT. Apoptosis and Caspase-3 activity were detected with flow cytometer and fluorometer. Apoptotic morphology was observed under fluorscent microscope. Results Survivin antisense oligodeoxynucleotide inhibited the cells＇ proliferation in a dose and time dependent manner. Compared with control, apoptotic rate increased, （18. 60 ± 3.46）% vs （1.35 ± 0.31） % and Caspase-3 activity increased by 77.99% after treatment with 400 nmol/L antisense oligodeoxynucleotide for 48 hours. Apoptosis with nuclear chromatin condensation and fragmentation as well as cell shrinkage and the formation of apoptotic bodies were observed. Conclusion Survivin antisense oligodeoxynucleotide is able to inhibit K562 cells＇ proliferation and induce apoptosis.

关 键 词：SURVIVIN 反义寡核苷酸 K562 凋亡 

分 类 号：R733.7[医药卫生—肿瘤]