食品污染微生物基因组DNA提取的简易方法  

A Easy Method for Extraction of Genomic DNA from the Microbe in the Contaminative Food

作　　者：魏兆军[1] 廖爱美[1] 贾如[1] 姜绍通[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2006年第12期410-413,共4页Food Science

基　　金：合肥工业大学创新团队基金资助项目

摘　　要：本文报道了直接抽提食品污染微生物的基因组DNA的方法。污染的食品溶于PBS缓冲液后,直接用含SDS的裂解液裂解微生物细胞。抽提出的微生物基因组DNA在23kb处有清晰条带,利用P1和P2扩增出16SrDNA基因约180bp的目的条带,克隆后随机测定一个阳性克隆的序列,分析表明我们测定的与细菌的KVD-1921-15菌株非常接近。In present study, the genomic DNA from the Microbe in the contaminative food was extracted directly. Firstly, the contaminative food was washed using PBIS. After centrifugation, the superior liquid was extracted in extraction buffer which containing SDS. The results showed that extracted genomic DNA was about 23kb. Using primers P1 and P2, the 180bp fragraent of 16SrDNA gene could be amplificated. Then, the PCR productions were cloned into T-vector. A positive clone was sequenced, which was similar to the sequence of the KVD-1921-15 stain from Bacteria.

关 键 词：基因组DNA 微生物 抽提 方法 

分 类 号：Q78[生物学—分子生物学]