Study of the interaction of Zn^(2+) with Aβ(10-21) by fluorescamine assay  

作　　者：Chao Feng Zhang Li Fan Pin Yang 

机构地区：[1]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China

出　　处：《Chinese Chemical Letters》2007年第1期97-98,共2页中国化学快报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(No.30470408).

摘　　要：Zinc may play a role as a co-factor in the pathogenesis of Alzheimer＇s disease （AD） through influencing the conformation and neurotoxicity of amyloid β-protein （Aβ）. Using the fluorescamine assay, we show for the first time that Zn^2＋ induced Aβ（10-21） aggregate in a concentration-dependent manner. These results indicate that Aβ（10-21） can be used as an in vitro model in Zn^2＋ - induced Aβ aggregation and that the region 10-21 to be the minimal fragment of zinc-binding domain of full length Aβ（1-42）.Zinc may play a role as a co-factor in the pathogenesis of Alzheimer＇s disease （AD） through influencing the conformation and neurotoxicity of amyloid β-protein （Aβ）. Using the fluorescamine assay, we show for the first time that Zn^2＋ induced Aβ（10-21） aggregate in a concentration-dependent manner. These results indicate that Aβ（10-21） can be used as an in vitro model in Zn^2＋ - induced Aβ aggregation and that the region 10-21 to be the minimal fragment of zinc-binding domain of full length Aβ（1-42）.

关 键 词：Aβ（10-21） FLUORESCAMINE Zn^2＋ AGGREGATION 

分 类 号：R749.16[医药卫生—神经病学与精神病学]