C57BL/6小鼠胆结石形成中固醇载体蛋白2的mRNA水平和胆固醇饱和指数增高  

mRNA of SCP 2 and cholesterol saturation index are increased during gallstone formation in C57BL/6 mice

作　　者：安国顺[1] 王海梅[2] 巴图乌拉[2] 王俊峰[2] 倪菊华[1] 李月廷[2] 祝学光[3] 

机构地区：[1]北京大学医学部生化与分子生物学系,北京100083 [2]北京中医药大学北京中西医结合医院普外科,北京100039 [3]北京大学人民医院普外科,北京100044

出　　处：《基础医学与临床》2006年第10期1108-1111,共4页Basic and Clinical Medicine

基　　金：首都医学发展科研基金(中医药)(2001年Ⅲ-19)

摘　　要：目的观察C57BL/6鼠胆囊结石形成过程中肝固醇载体蛋白2(SCP2)mRNA水平及胆囊胆汁中胆固醇饱和指数的变化。方法C57BL/6鼠20只,分为结石组和对照组。对照组喂普通饲料,结石组采用1%的胆固醇饮食4周;RT-PCR方法检测肝SCP2 mRNA水平的变化;全自动生化仪检测胆脂,并计算胆固醇饱和指数。结果胆囊结石组肝SCP2 mRNA水平显著升高,胆汁胆固醇饱和指数亦显著增加。结论肝SCP2的表达及胆汁CSI的增高呈正相关。Objective To analyze the level of hepatic sterol carrier protein 2 （SCP2） mRNA and bile cholesterol saturation index（CSI） during gallstone information in C57BL/6 mice. Methods Twenty C57BL/6 mice were divided into control group and gallstone group. The gallstone group mice were fed with lithogenic diet （ containing 1% cholesterol） for 4 weeks. The mRNA level of SCP2 in liver tissue was measured by RT-PCR. The content of biliary lipids and CSI was analyzed by Automatic Biochemistry Analyzer. Results The level of SCP2 mRNA and bile CSI were significantly increased in gallstone group. Conclusion A positive correlation between expression of liver SCP2 and bile CSI was found during gallstone formation.

关 键 词：固醇载体蛋白2 胆固醇饱和指数 胆石形成 

分 类 号：R575.62[医药卫生—消化系统] Q548.1[医药卫生—内科学]