细胞周期调控因子与下咽癌生物学行为相关性初步研究  

Preliminary Study of the Relationship between Cell Cycle Regulatory Factors and Biological Behavior of Hypopharyngeal Carcinoma

作　　者：陈万军[1] 李梅[2] 王兴武[1] 王超[1] 邹淑娟[1] 陶阳[1] 王天铎[2] 

机构地区：[1]山东省肿瘤防治研究院头颈科,济南市250117 [2]山东大学齐鲁医院耳鼻喉科

出　　处：《中国肿瘤临床》2007年第1期26-29,共4页Chinese Journal of Clinical Oncology

基　　金：山东省自然科学基金资助(编号:Y2004C30)

摘　　要：目的:探讨细胞周期调控因子与下咽癌临床生物学行为之间的关系。方法:取下咽鳞状细胞癌手术标本53例,癌旁正常粘膜标本11例,应用免疫组化SP法检测P27Kip1、CyclinD1、CDK4在下咽癌组织及正常粘膜中的表达。结果:P27Kip1、CyclinD1、CDK4均为弥漫性细胞核表达。P27Kip1在下咽癌中的表达低于正常粘膜,且表达与N分级明显相关(P<0.05);CyclinD1在下咽癌中的表达高于正常粘膜,且表达与T分级明显正相关(P<0.05);CDK4在下咽癌中的表达高于正常粘膜,且表达与T分级、N分级明显正相关(P<0.01、P<0.05)。P27Kip1与CyclinD1,P27Kip1与CDK4在下咽癌组织中多呈反向表达,CyclinD1与CDK4多呈同向表达。结论:CyclinD1、CDK4与P27Kip1在下咽癌发病机制中共同作用,对预后有一定的参考价值。Objective： To determine the relationship between cell cycle regulatory factors and biological behavior of hypopharyngeal squamous cell carcinoma. Methods： Immunohistochemical staining by specific monoclonal anti-p27/Kipl protein, anti-cyclin D1 protein and anti-CDK4 protein was performed using the S-P method in 53 cases of hypopharyngeal squamous cell carcinoma and 11 cases of normal epithelium. Results： All of the positive staining for Cyclin D1, CDK4 and p27/Kipl in hypopharyngeal squamous cell carcinoma showed suffusing expression in the nucleolus. There was a significant difference in expression between the cancerous cells and normal epithelium. The negative lymph node group had a significantly higher immunoreactivity for p27/Kipl than the lymph node positive group （P〈 0.05）. Cyclin D1 expression was related to T grade （P〈0.05）. A significant positive correlation was found between CDK4 expression and T and N grade （P〈0.01, P〈0.05）. The reverse pattern was found in p27/Kipl-Cyclin D1 and p27/Kipl-CDK4. On the other hand, unidirectional expression was found for Cyclin D1 and CDK4. Conclusion： Cyclin D1, CDK4 and p27/Kipl each play a role in hypopharyngeal squamous cell carcinoma progression and together can predict the probable disease course and outcome.

关 键 词：下咽 鳞状细胞 癌 细胞周期 CYCLIN D1 CDK4 P27^KIPL 

分 类 号：R739.6[医药卫生—肿瘤]