溶胶-凝胶法固定化α-淀粉酶及其固定床运行研究  

Study on Sol-gel Encapsulated α-Amylase and Its Utilization in Fixed Bed

作　　者：司志斌[1] 高春光[1] 张铁明[1] 赵永祥[1] 

机构地区：[1]山西大学化学化工学院,山西太原030006

出　　处：《化学与生物工程》2007年第1期36-37,共2页Chemistry & Bioengineering

基　　金：山西省科委攻关项目(041122)

摘　　要：以正硅酸乙酯为硅源、醋酸为水解催化剂,采用溶胶-凝胶法,室温下制得包埋α-淀粉酶的二氧化硅凝胶颗粒。模拟固定床的条件,测定了固定化α-淀粉酶的重复使用回收活性及米氏常数,结果表明其重复使用40次后仍保留30%以上的初始活性。Using ethyl orthosilicate as silica source and acetic acid as hydrolysis catalyst,the SiO2 gel particles encapsulating α-amylase were prepared through sol-gel method at room temperature. Under analog fixed bed conditions,the recover activity of encapsulated α-amylase reused and the Km were determined. The results showed that α-amylase kept over 30% initial activity after used for 40 times.

关 键 词：溶胶-凝胶法固定化酶 Α-淀粉酶 米氏常数 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]