人精子染色体结构畸变与苯妥英钠的影响(英文)  

Structural aberration of human sperm chromosomes and the effects of phenytoin sodium

作　　者：蒙华庆[1] 

机构地区：[1]重庆医科大学附属第一医院心理卫生中心,重庆市400016

出　　处：《中国组织工程研究与临床康复》2007年第4期798-800,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(39270810)~~

摘　　要：背景:当各级生殖细胞DNA受到损伤而发生突变时,均可能通过受精卵遗传给后代,苯妥英钠作为传统抗癫痫药物,已被许多研究证实其对体细胞具有诱变效应,而是否对生殖细胞也具有诱变作用,并通过受精卵遗传给后代,尚需进一步研究。目的:检测苯妥英钠对人精子染色体的诱变效应。设计:以离体人精子染色体为观察对象的随机对照观察。单位:重庆医科大学附属第一医院心理卫生中心。材料:苯妥英钠购自Sigma公司,人精液标本取自近半年内未接触过理化诱变因子的健康成年男性。鼠卵取自6～8周龄的雌金黄地鼠,用含0.3%人血清白蛋白的BWW液培养基洗涤精卵和受精,在含3.5%血清白蛋白的BWW液培养基中使精子获能,受精后培养基用含10%地鼠血清的卵培养基。方法:将精子洗涤、离心、获能后,制作成5mL精子悬液,平均分置于5支离心管中,一支加入终浓度为40mg/L的平阳霉素作为阳性对照,一支不加任何试剂作为空白对照,其余3支分别加入终浓度为10mg/L、20mg/L、40mg/L的苯妥英钠。按常规方法制作无透明带的地鼠卵后,平均分为5等份,分别与上述5组精液混合,使地鼠卵受精,并用受精的地鼠卵制备人精子染色体。采用染色体结构畸变精子率(简称畸精率)和断裂数2个指标,应用人精子染色体离体测试系统检测不同浓度苯妥英钠对人精子染色体的诱变作用,并与平阳霉素40mg/L组和空白对照组比较。主要观察指标:各染色体精子染色体结构畸变情况,结构畸变精子率和断裂数。结果:①染色体结构畸变:不同浓度苯妥英钠组、阳性对照组和空白对照组均显示染色体断裂和单体断裂、断片、相互易位、双着丝粒体、环状染色体等多种精子染色体结构畸变,尤以苯妥英钠40mg/L组和平阳霉素40mg/L组更为显著。②染色体结构畸变精子率和染色体断裂数:不同浓度苯妥英钠组和平阳霉素4BACKGROUND ： If the generative cell DNA of each grade is damaged or mutated, it is possible to transmit to the further generations by means of fertilized ova. As a traditional antiepileptic, the mutagenic effects of phenytoin sodium on somatic cells had been confirmed by many researches, but it is still unknown whether phenytoin sodium has the mutagenic effects on generative cells. OBJECTIVE： To detect the mutagenic effects of phenytoin sodium on human sperm chromosomes DESIGN：In present study we measured human sperm chromosomes in vitro by means of randomized control observation.SETTING： Mental Health Center of the First Affiliated Hospital, Chongqing University of Medical Sciences. MATERIALS： Phenytoin sodium was purchased from Sigma Company. The human sperms were collected from the healthy males who had not contacted with any physicochemical mutagen within recent 6 months. Ova were collected from female golden hamsters of 6-8 weeks, which were purchased from Shanghai Institute of Family Planning Science. Medium for washing sperms and ova was the BWW solution containing 0.3% human serum albumin （HSA）; Medium for capacitation was the BWW solution containing 3.5% HSA; Medium for post-fertilization was an oval one containing 10% hamster serum. METHODS： After washing, centrifugation and capacitation, the sperms were made into suspension and dispended into 5 centrifuge tubes （5 mL each）： Bleomycin A5 （40 mg/L） was added in the first tube as positive control group, phenytoin sodium （10, 20 and 40 mg/L） were added in three tubes respectively, another tube did not contain any reagent as blank control. Hamster ova without pellucid zone were prepared, and equally divided into five portions, which were mixed with the above-mentioned sperms in the five groups respectively, so as to make the hamster ova fertilize, finally human sperm chromosomes were prepared with the fertilized hamster ova. The rate of chromosomal structural aberration （rate of aberrant sperm） and number of chromosomal bre

关 键 词：苯妥英钠 人 精子 染色体结构 染色体畸变 

分 类 号：R596.1[医药卫生—内科学]