大口黑鲈主要消化器官蛋白酶活力的研究  

Protease activities in digestive organ in largemouth bass(Micropterus salmoides)

作　　者：关胜军[1] 吴锐全[2] 谢骏[2] 王广军[2] 谢一荣[1] 叶富良[1] 陈刚[1] 

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]中国水产科学研究院珠江水产研究所,广东广州510380

出　　处：《大连水产学院学报》2007年第1期32-36,共5页Journal of Dalian Fisheries University

基　　金：广东省科技计划项目资助(2004B20301005);中国水产科学研究院人才基金资助项目

摘　　要：从酶学分析角度研究了大口黑鲈Micropterus salmoides主要消化器官蛋白酶的活力分布,以及不同温度、pH和底物浓度条件下其胃、幽门盲囊、肠中蛋白酶的活力变化。结果表明:不同消化器官中蛋白酶的活力存在明显差异,由高到低依次为胃>幽门盲囊>前肠>中肠>后肠;蛋白酶的活力随温度和pH变化呈现一定的规律性,不同消化器官蛋白酶的最适温度、pH依次为胃(40℃,2.4)、幽门盲囊(55℃,7.6)、肠(45℃,8.0),蛋白酶的最适温度高于体温,胃蛋白酶在强酸环境下活力最大,幽门盲囊与肠在中性和偏碱条件下活力最大;蛋白酶的活力与底物浓度有关,最大活力的底物酪素浓度依次为胃20 g/L、幽门盲囊20 g/L、肠10 g/L。Protease activities were determined in different parts of digestive organs in largemouth bass （Micropterus salraoides） at different temperature, pH and casein concentration by means of enzymological analysis. The results showed that the protease activities in different parts of digestive organs were arranged in a descent order as： stomach 〉 pyloric caeca 〉 foregut 〉 midgut 〉 hindgut. The protease activities changed with variations in temperature, pH and casein concentration in different organs. The optimum temperatures and pH were （40℃, 2.4 ） for stomach, （55℃ ,7.6） for pyloric caeca（45℃ ,8.0） for gut. The optimum temperature for protease activity was higher than that in the fish body. The highest activity of protease occurred in strong acidic condition in stomach, but in neutral or weak alkaline condition in pyloric caeca and gut. The activity of the protease also changed with concentrations of the casein, the optimum casein concentration of 20 g/L for stomach. 20 g/L for pyloric caeca, and 10 g/L for gut.

关 键 词：大口黑鲈 蛋白酶 温度 PH 酶活力 

分 类 号：S965.211[农业科学—水产养殖]