菊粉酶中色氨酸残基的化学修饰及其荧光光谱  

Tryptophan Modification and Fluorescence Spectrum of Inulinase

作　　者：刘仙[1] 高国粉[1] 杨丽[1] 何潇潇[1] 孟哲[1] 滕利荣[1] 

机构地区：[1]吉林大学生命科学学院,长春130012

出　　处：《高等学校化学学报》2007年第1期103-105,共3页Chemical Journal of Chinese Universities

基　　金：吉林大学2005年学生科技创新基金资助

摘　　要：Tryptophan(Trp) residues in inulinase were modified by chemical reagent N-bromossuccinimide(NBS).The results of Spande′s method indicate that there were seventeen Trp residues in inulinase and five of them were located on the surface of the enzyme.Three of these Trp residues were none-essential residues which showed the fastest rate by Zhou′s plot.Two relative faster reacting residues were both essential for the activity of the enzyme.The other twelve were the slowest or none-reactive residues for the reaction.The study on fluorescence quenching of inulinase shows that KI could not quench all of the fluorescence from Trp residues in inulinase which indicate that there are two kinds of Trp residues in inulinase acrylamide(Acr),a polarized quencher without electronic charge could quench almost all of the fluorescence from Trp residues in inuoinase while there are still seventy percernt of the activity of the enzyme left.The collisional quenching constants(K_D) of inulinase at different concentrations of Acr were calculated in terms of Stern-Volmer equation.Tryptophan（Trp） residues in inulinase were modified by chemical, reagent N-bromossuccinimide （NBS）. The results of Spande＇s method indicate that there were seventeen Trp residues in inulinase and five of them were located on the surface of the enzyme. Three of these Trp residues were none-essential residues which showed the fastest rate by Zhou＇s plot. Two relative faster reacting residues were both essential for the activity of the enzyme. The other twelve were the slowest or none-reactive residues for the reaction. The study on fluorescence quenching of inulinase shows that KI could not quench all of the fluorescence from Trp residues in inulinase which indicate that there are two kinds of Trp residues in inulinase acrylamide（ Acr）, a polarized quencher without electronic charge could quench almost all of the fluorescence from Trp residues in inuoinase while there are still seventy percernt of the activity of the enzyme left. The collisional quenching constants（KD） of inulinase at different concentrations of Acr were calculated in terms of Stern-Volmer equation.

关 键 词：菊粉酶 色氨酸残基 化学修饰 荧光猝灭 

分 类 号：Q556[生物学—生物化学]