体外法评定甘露寡糖和甜菜汁对肠道微生物发酵的影响  

Effect of mannan-oligosaccharide and sugar beet pulp on intestinal microbial fermentation in vitro

作　　者：杭苏琴[1] 毛胜勇[1] 于卓腾[1] 朱伟云[1] 

机构地区：[1]南京农业大学消化道微生物研究室,江苏南京210095

出　　处：《南京农业大学学报》2007年第1期79-83,共5页Journal of Nanjing Agricultural University

基　　金：国家973计划项目(2004CB117500);国家杰出青年科学基金(30025034)

摘　　要：以厌氧收集的仔猪25日龄断奶当天回肠、结肠食糜作为接种物,甘露寡糖(MOS)和甜菜汁(SBP)为底物,体外发酵48 h,测定产气量及挥发性脂肪酸(VFA)浓度,以研究MOS和SBP对仔猪肠道微生物影响。结果表明,MOS组和SBP组产气量、产酸量都高于对照组,且呈剂量效应。在回肠,MOS各组乙酸比例显著低于对照,丙酸和丁酸比例显著高于对照;SBP组以乙酸发酵为主,但其比例随剂量增加而下降,而丁酸比例则随之显著上升。在结肠,MOS和SBP各组的丁酸比例均显著高于对照;MOS组乙酸比例随剂量增加而下降,SBP组仍以乙酸为主,但剂量效应不明显。以上结果提示,MOS和SBP具有促进肠道微生物发酵,提高肠道丁酸比例的潜在益生作用。Ileal and colon digesta as sources of inocula were collected from piglets of 25 day-old on day 1 of weaning. 48 hours fermentation of mannan-oligosaccharide （MOS） and sugar beet pulp （SBP） as substrates were investigated in vitro to determine the effect on gastrointestinal microbiota. Gas production and volatile fatty acids （VFA） were analysed. The results showed that the production of gas and VFA was significantly increased with the increasing concentration of MOS and SBP in cultrure medium. For ileum inoeula, all MOS treatments showed acetic acid proportion significantly lower than the control, while propionic and butyric acids proportions significantly higher than the control; in SBP treatments, acetic acid was still the major acid, but its value decreased while butyric acid increased as the substrate concentration increased. For colon inocula, all MOS and SBP treatments showed higher butyric acid proportion than the control; the proportion of acetic acid decreased as MOS concentration increased, but no dose effect was observed with SBP though acetic acid was still the major acid. The results suggested that MOS and SBP could promote piglet gasintestinal microbial fermentation, especially the production of butyric acid, implying their potential as proprotion.

关 键 词：甘露寡糖 甜菜汁 断奶仔猪肠道食糜 体外发酵 产气量 挥发性脂肪酸 

分 类 号：S811.6[农业科学—畜牧学]