膳食钙对高脂饮食大鼠肥胖形成的影响  

Effect of dietary calcium on the obesity of rats fed with highfat diet

作　　者：罗楠[1] 秦锐[1] 

机构地区：[1]南京医科大学第二附属医院儿童保健研究中心,210011

出　　处：《江苏医药》2007年第2期162-164,共3页Jiangsu Medical Journal

基　　金：江苏省卫生科研项目(H200321)

摘　　要：目的研究膳食钙摄入对高脂饮食大鼠肥胖形成的影响。方法30只SD大鼠标准饲料饲养10d后,随机分为标准饲料(A)组(1.30%钙),高脂饲料(B)组(1.57%钙)和高脂加钙饲料(C)组(2.57%钙),饲养6周后处死,称量各组大鼠体重、体脂,用ELISA法测血清钙调激素甲状旁腺素(PTH)和1,25-(OH)2D3水平,用RT-PCR检测白色脂肪组织(WAT)中VitminD受体(VDR)、脂肪酸合成酶(FAS)、解偶联蛋白2(UCP2)基因mRNA表达水平。结果(1)B组体重、Lee′s指数、脂肪湿重显著高于A组和C组(P<0.05);(2)C组的血清钙调激素水平均显著低于B组(P<0.05);(3)C组VDR和FAS基因mRNA表达水平均显著低于B组(P<0.05),C组UCP2基因mRNA表达水平均显著高于B组(P<0.05)。结论高钙膳食可能通过降低血清钙调节激素水平,影响脂肪代谢和钙代谢,从而减少高脂饮食大鼠体重和脂肪重量的增加。Objective To investigate the effect of dietary calcium on the obesity of rats fed with high fat diet. Methods After ten days of free access to a standard diet, 30 SD rats were randomly divided into 3 groups with 10 rats each. The rats in groupA were fed with standard diet（1.30% calcium）, those in group B with high fat and normal calcium diet（1.57% calcium）, and those in group C with high fat and high calcium diet（2. 57% calcium）. At the end of 6 weeks, the rats were sacrificed. The body weight and the wet weight of fat pad were weighted. Serum calcitrophic hormones PTH and 25-（OH）2D3 were measured with ELISA method. And the levels of FAS, VDR and UCP2 mRNA in white adepose tissuc were measured with RT-PCR. Results The body weight, Lee＇s index and wet weight of fat pad were higher in group B than those in group A or C（P〈0. 05）. The levels of serum PTH and 25-（OH）2D3 in group C were lower than those in group B（P〈0. 05）. VDR mRNA expression in group C was lower than that in group B（P〈0. 05）. FAS mRNA expression in group C was lower than that in group B（P〈0. 05）. UCP2 mRNA expression in group C was higher than that in group B（P〈0, 05）. Conclusion High calcium diet may influence fat and calcium metabolism, which can result in reducing the body weight and fat pad of the rats fed with high fat diet, indicating that dietary calcium may play a role in the prevention of obesity.

关 键 词：膳食钙 高脂饮食 肥胖 脂肪代谢 钙代谢 

分 类 号：R151[医药卫生—营养与食品卫生学]