高效液相色谱法测定脱臭馏出物深加工物中的维生素E和甾醇  

Determination of Vitamin E and Sterols in Refined Products from Deodorizer Distillate by High Performance Liquid Chromatography

作　　者：邵平[1] 姜绍通[1] 潘丽军[1] 陈晓燕[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2007年第1期229-231,共3页Food Science

基　　金：安徽省自然科学基金项目(03041302)

摘　　要：建立了一种分析菜籽油脱臭馏出物深加工物中维生素E和甾醇含量的分析方法。该方法采用反相C18柱,流动相为96:4(V/V)的甲醇水混合液,流速1.0ml/min,柱温30℃,二极管阵列检测,外标法定量。结果表明所采用的标准曲线有良好的线形关系、回收率,方法精密度良好。将该方法用于脱臭馏出物深加工物中维生素E和甾醇的测定,简单快速,结果准确、可靠。A high performance liquid chromatographic method for the determination of vitamin E and sterols has been established.Separation was carried out on a C18 column （Atlantics column） at 30℃ with mobile phase of methanol and water（96：4, V/V）, and PDA detection for vitamin E at 292nm and for sterols at 205nm. The experimental results showed that there is a good linear relationship between the peak area and the concentration of components. The average recoveries and relative standard deviation were well showed. The results showed that the method is simple,rapid and accurate for dcterminetion of vitamin E and sterols in deodorizer distillate.

关 键 词：脱臭馏出物 VE 甾醇 高效液相色谱 

分 类 号：O657.72[理学—分析化学]