一种快速检测分离溶藻细菌方法的初探  

A Methodological Study on Rapid Identification and Isolation of Algicidal Bacteria

作　　者：吕伟英[1] 赵以军[1] 周瑞[1] 吴广涛[2] 胡蝶[1] 吴刚[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079 [2]华中农业大学生命科学技术学院,武汉430070

出　　处：《微生物学通报》2007年第1期119-122,共4页Microbiology China

基　　金：国家自然科学基金(No.30370062);武汉市重点科技攻关项目(No.20026002097)

摘　　要：传统的细菌培养基对铜绿微囊藻具有毒性作用。会大大影响溶藻细菌的筛选效率和准确性。通过基本培养基各成分对铜绿微囊藻DS的作用研究发现,培养基中的葡萄糖成分对藻有抑制作用,并且这种抑制作用与培养基中的葡萄糖浓度密切相关,当培养基中葡萄糖的浓度在0．1～0．4g/L时,藻细胞生长受到抑制,当用柠檬酸三钠取代葡萄糖后,铜绿微囊藻在改良后的培养基中生长正常,与对照组相比无显著性差异(P＜0．05)。用改良的培养基富集水样中的溶藻微生物,并用此培养液直接感染宿主藻,一周内即可初步快速检测是否含有溶藻细菌。此种方法既排除了培养基的干扰因素,又迅速增加了溶藻细菌的生物量,并可大量收集细菌分泌的胞外物质,为溶藻细菌尤其以分泌物质溶藻的细菌的初步筛选提供了一条快捷、有效的途径。In order to increase the efficiency of algicidal bacteria isolation , The components of minimal medium for isolation of algicidal bacteria were tested for the effection growth of Microcystis aeruginosa DS and a modified minimal medium was established.. The results indicated that glucase at the concentration of 0. 1-0. 4g/L in minimal medium inhibited the growth of Microcystis aeruginosa DS , and a modified minimal medium was developed using sodium citrate instead of glucoso as carbon sourso. On modified medium algicidal bacteria could be successfully isolated directly from field sample in 1 week. Microcystis aeruginosa DS could grow well on modified medium and the algicidal materials were secreted into medium by bacteria and the growth medium could be directly used for algicidal test.

关 键 词：溶藻细菌 微囊藻 葡萄糖 快速检测 

分 类 号：X172[环境科学与工程—环境科学]