阿托伐他汀钙对大鼠脑缺血再灌后NF-kBp56表达的影响  

Effects of atorvastatin on the expression of NF-kBp56 after cerebral ischemia/reperfusion injury in rats

作　　者：谷文萍[1] 肖慧[1] 李伟[1] 刘福中[1] 王妮妮[1] 宋小明[1] 杨期东[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008

出　　处：《卒中与神经疾病》2007年第1期3-5,9,共4页Stroke and Nervous Diseases

摘　　要：目的探讨阿托伐他汀钙对大鼠脑缺血再灌后脑组织中NF-kBp56mRNA及蛋白表达的影响。方法采用大脑中动脉线栓法制备局灶性脑缺血再灌注模型,参考Longa的5分制法在大鼠麻醉清醒后进行评分,应用原位杂交和免疫组化法检测NF-kBp56mRNA及蛋白表达。结果大鼠脑缺血再灌注后缺血脑组织中NF-kBp56mRNA和蛋白表达增加(P<0.01),24h达高峰;给予阿托伐他汀钙干预后能减少缺血脑组织中NF-kBp56mRNA和蛋白表达(P<0.05),降低神经功能缺损评分(P<0.05)。结论阿托伐他汀钙能抑制大鼠脑缺血再灌注后脑组织中NF-kBp56mRNA及蛋白表达,减轻缺血再灌损伤。Objective To investigate whether atorvastatin has impact on the expression of NF-kBp56 in the brain tissue after ischemia/reperfusion（I/R） injury. Methods Rats were subjected to cerebral I/R injury through the occlusion of the middle cerebral artery. Neurological deficits were determined by Longa＇s score. The expression of NF-kB was detected by immuno-histochemical analysis, and the expression of NF- kBp65mRNA was detected by in situ hybridization method. Results The expression of NF-kBp65 and NF- kBp65 mRNA in the ischemic territory increased after cerebral ischemia（P〈0. 01 ）, peaking at 24 h after ischemia. Atorvastatin could decrease the expression of NF-kBp65 and NF-kBp65mRNA（P〈0. 05） and improve the Longa＇s score （P〈0. 05）. Conclusions Atorvastatin-calcium protect the brain tissue against ischemia reperfusion injury through inhibiting the expression of NF-kBp56 and NF-kBp65mRNA after focal cerebral ischemia reperfusion in rats.

关 键 词：阿托伐他汀钙 脑缺血再灌注 炎症反应 NF-kBp56 

分 类 号：R743[医药卫生—神经病学与精神病学]