分解酒糟生物质的纤维素酶生产菌的筛选研究  

Study on the Screening of Cellulase Strains for Distiller Biomass Degradation

作　　者：江龙法[1] 钱志刚[1] 夏泽华 李加坤[1] 张红 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005 [2]南通水明楼酒业有限公司,江苏南通226500

出　　处：《淮海工学院学报（自然科学版）》2006年第4期51-54,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：江苏省科技厅科技攻关项目(BE2004305)

摘　　要：从白酒糟采集的样品中分离到15株菌，经过刚果红染色，初筛得到产纤雏素酶酶活较高的5株菌。纤维素酶和滤纸酶活研究结果表明，这5株菌在分泌纤维素酶活力及降解滤纸能力方面均较强，其分解纤维素的酶活最高达到11．9U／mL，而分解滤纸的酶活力则最高达到6．4U／mL。最适产酶条件为：发酵时间72-96h，发酵温度25-30℃。对酒糟发酵减重最高达18%。15 strains were isolated from samples collected from distiller grains. Congo red staining method was adopted to obtain 5 strains with powerful CMCase-producing abilities. Enzymatic assays on CMCase and FPase activities of these 5 strains were performed. Results showed that CMCase was 11.9 U/mL and the FPas was 6.4 U/mL. The optimum conditions for producing this cellulase had been verified as follows： fermentation temperature 25-30 ℃ and fermentation time 72-96 h, and the highest rate can be amounted to 18%.

关 键 词：纤维素分解菌 酒糟生物质 酶活力 

分 类 号：Q814.1[生物学—生物工程]