金钱松内生真菌抗植物病原真菌活性的研究  

Study on the Antifungal Activity of Endophytes of Pseudolarix kaempferi Gord

作　　者：何佳[1] 陈钧[1] 赵启美[1] 祁红兵[1] 

机构地区：[1]江苏大学生物与环境工程学院

出　　处：《河南农业科学》2007年第2期46-49,共4页Journal of Henan Agricultural Sciences

基　　金：江苏省2004年度博士研究生创新计划项目(xm04-57)

摘　　要：首次对金钱松内生真菌进行了分离,并以稻瘟霉(Pyriculariaoryza)P-2b活性筛选体系为筛选模型,以水稻纹枯病菌(Corticium sasakii)AS3.2872、亚麻刺盘孢(Colletotrichum lini)AS3.4486、禾谷镰孢(Fusarium graminearum)AS3.4598和棉灰星盘多毛孢菌(Pestalotia gossypii Hori)等4种常见植物病原真菌为指示菌,研究了金钱松内生真菌抗植物病原真菌活性菌株的抗菌能力。结果表明,金钱松内存在着大量具有抗稻瘟霉P-2b活性的内生真菌,JJ18,JJ28,JJ314和JJ323等4株活性菌株对指示菌表现出强烈的广谱抗菌能力,其中JJ314,JJ323可诱导稻瘟霉P-2b菌丝呈现明显的念珠状变形现象,表明这2株活性菌株代谢产物中可能含有低毒性抗真菌成分。金钱松内生真菌是潜在的杀菌生物农药资源。Objective： To study antifungal activity of endophytes from Pseudolarix kaempferi Gord. Method： The endophytes from P.seudolarix kaempferi Gord were separated for the first time. Antifungal endophytes were screened by Pyricularia oryzae P-2b model, and the activity of endophytes against Corticium sasakii AS3. 2872, Colletotrichum lini AS3. 4486, Fusarium graminearum AS3. 4598 and Pestalotia gossypii Hori were studied. Result： There were many endophytes against Pyricularia oryzae P-2b in Pseudolarix kaempferi Gord. JJ18, JJ28, JJ314 and JJ323 had broad-spectrum intense antifungal activity to the four indicators. JJ18 and JJ28 could induced the formation of characteristic beads to the growing Pyricularia oryzae P-2b hyphae, indicating that it was possible for the two strains to produce antifungal agents of weak toxicity. Conclusion： The endophytes from Pseudolarix kaempferi Gord were a potential resource for antifungal agent.

关 键 词：金钱松 内生真菌 稻瘟霉 植物病原真菌 

分 类 号：S949.32[农业科学—水产养殖]