Differential expression of βig-h3 in human hepatoma cell lines  

作　　者：郭卫平 周宏伟 蒋建利 张洪新 

机构地区：[1]Department of Interventional Radiation,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China [2]Cell Engineering Research Centre,Fourth Military Medical University,Xi'an 710033,China

出　　处：《Journal of Medical Colleges of PLA(China)》2007年第1期8-11,共4页中国人民解放军军医大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No. 30200144)

摘　　要：Objective：To observe the differential expression of TGF-β-induced gene human clone 3 （βig- h3） in human hepatoma cell lines. Methods：Human hepatoma cells HHCC, 7721, T7721 constructed by stably transfectiug HAb18G/CD147 cDNA into 7721 and normal human liver cell QZG were cultured as previously. RT-PCR and western blot were used to investigate the differential expression of βig-h3 in human hepatoma cell lines and normal liver cell. Results ：The results of RT-PCR suggested that the expression of βig-h3 mRNA in human hepatoma cells was higher than that in normal human liver cell QZG（P〈0.01）, and its expression level in human hepatoma cells in turn was HHCC〉T7721〉7721. Moreover, the similar results of βig-h3 protein expression were testified by western blot. Conclusion：This study demonstrates that the expression of βig-h3 in hepatoma cell lines is higher than that in normal liver cell QZG, which provides a sound basis for exploring the function of βig-h3 in processes of adhesion and metastasis of human hepatoma cells.Objective:To observe the differential expression of TGF-β-induced gene human clone 3 (βig-h3) in human hepatoma cell lines. Methods: Human hepatoma cells HHCC. 7721. T7721 constructed by stably transfecting HAbl8G/CD147 cDNA into 7721 and normal human liver cell QZG were cultured as previously. RT-PCR and western blot were used to investigate the differential expression ofβig-h3 in human hepatoma cell lines and normal liver cell. Results:The results of RT-PCR suggested that the expression ofβig-h3 mRNA in human hepatoma cells was higher than that in normal human liver cell QZG(P< 0. 01), and its expression level in human hepatoma cells in turn was HHCC>T7721>7721. Moreover, the similar results ofβig-h.3 protein expression were testified by western blot. Conclusion: This study demonstrates that the expression ofβig-h3 in hepatoma cell lines is higher than that in normal liver cell QZG, which provides a sound basis for exploring the function ofβig-h3 in processes of adhesion and metastasis of human hepatoma cells.

关 键 词：βig-h3 HEPATOMA HAB18G/CD147 ADHESION 

分 类 号：R735.7[医药卫生—肿瘤] R73-37[医药卫生—临床医学]