Notch1、Delta、NF-κB在肝癌前病变大鼠中的表达及清肝化瘀方药的调节作用  

Expression of Notch1、Delta、 NF-κB in rats hepatic precancerous lesion and the regulating effection of the Qinggan Huayu Formula

作　　者：杨志云[1] 姚树坤[2] 殷飞[2] 修贺明[3] 

机构地区：[1]河北医科大学,河北石家庄050017 [2]河北医科大学第四附属医院消化内科,河北石家庄050011 [3]白求恩国际和平医院中心实验室,河北石家庄050082

出　　处：《中成药》2007年第2期175-178,共4页Chinese Traditional Patent Medicine

基　　金：河北省自然科学基金资助项目(No:C2006000852)

摘　　要：目的:探讨清肝化瘀方药阻断大鼠肝癌前病变的作用机制。方法:SD大鼠随机分为4组:模型组(A组)、清肝化瘀方药等效剂量组(B组)、清肝化瘀方药大剂量组(C组)、对照组(D组),分别用酶组化及免疫组化方法检测γ-谷胺酰转肽酶(γ-GT酶)、Notch1、Notch1配体Delta、NF-κB的表达变化。结果:与D组比较,A组γ-GT酶、Notch1、Delta、NF-κB表达水平显著升高(P<0.01),并且NF-κB与Notch1、Delta之间呈显著正相关(r值分别为0.865、0.742,P<0.01)。与A组比较,C组γ-GT酶、Notch1、Delta、NF-κB表达水平显著下调(P<0.01)。结论:清肝化瘀方药可保护肝细胞阻断癌变,其机制可能与下调Notch1、Delta、NF-κB有关。AIM： To investigate the mechanism of Qinggan Huayu Formula intervening rat＇s hepatic precancerous lesion. METHODS： SD rats were randomly divided into 4 groups： model group （group A）, moderate-dose Qinggan Huayu Formula group （group B ）, high-dose Qinggan Huayu Formula （group C ）, normal control group （group D）, and to detect the expression of γ-GT, Notchl ,Delta, NF-kB by enzyme-histochemisty and immunohistochemistry methods. RESULTS ： Compared with group D , the expressions of γ-GT, Notchl, Delta, NF-kB in group A were singnificanfly increased （ P 〈 0.01 ）, There were singnificantly positive correlations among NF-kB, Notchl and Deha（P 〈0.01 ） ,Compared with group A expressions of γ-GT,Notchl ,Delta, NF-kB in group C was singnificantly increased（P 〈0.01 ）. CONCLUSION： Qinggan Huayu Formula can protect from hepatic precancerous lesion,the mechanism was related to regulate the expressions of Notch1 ,Delta and NF-kB.

关 键 词：清肝化瘀方药 肝癌前病变 NOTCH1 DELTA NF-KB 

分 类 号：R284[医药卫生—中药学]