用Beckman　121MB型氨基酸分析仪快速分析含硫氨基酸和色氨酸  

Rapid Analysis of Cysteic Acid,Methionine Sulfone,and Tryptophan by Beckman 121MB Amino Acid Analyzer

作　　者：曹让[1] 张林生[1] 

机构地区：[1]西北农业大学实验中心

出　　处：《氨基酸和生物资源》1996年第4期34-36,共3页Amino Acids & Biotic Resources

摘　　要：蛋白质中胱氨酸、蛋氨酸和色氨酸在用盐酸水解时损失较大，常用过甲酸氧化法和碱水解法处理。采用文中方法分析时间短，分辨率高，半胱磺酸、甲硫氨酸砜和色氨酸的保留时间分别为５．３、１１．６和２０．４分钟。每分析一个样品需２６分钟，且基线平稳、重复性好、色谱图清晰。Cystine.Methionine and Tryptophan in protein is easier destroyed by HC1 hydrolysis.To protect these aminoacids from degradation,protein must be oxidized with performic acid and hydrolysed with NaOH before analysis The methols described in this paperr is a raprd method with high resolution.The retention time of cysteic acid,Methionine sulfone,and Tryptophan is 5.3,11.6,and 20.4 minutes,respectively.It took 26 minutes to analyse a sarnple.

关 键 词：氨基酸 半胱磺酸 色氨酸 氨基酸分析仪 

分 类 号：Q517.03[生物学—生物化学]