神经生长因子对大鼠脑缺血再灌注损伤的保护作用  

The protective effect of nerve growth factor on acute ischemia reperfusion injured rats

作　　者：李志坚[1] 王益光[2] 孙嘉斌[2] 王玉良[2] 唐可欣[2] 张薇[1] 郭顺生[1] 

机构地区：[1]潍坊医学院机能实验室,山东潍坊261042 [2]潍坊医学院生理学教研室

出　　处：《中国康复医学杂志》2007年第2期107-108,132,共3页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:探讨神经生长因子(NGF)对大鼠脑缺血再灌注损伤的保护作用及其机制。方法:采用大脑中动脉内栓线阻断法(MCAO)造成大鼠局灶性损伤模型,同时给予NGF治疗,观测神经评分改变,计算脑组织梗死灶,测定脑组织含水量以及一氧化氮合酶(NOS)活动的变化。结果:NGF治疗组神经评分明显降低(P<0.05);与脑缺血再灌注组比较,NGF治疗组脑缺血梗死区缩小,脑组织含水量和NOS活性明显降低(P<0.05)。结论:NGF具有保护脑缺血再灌注损伤的作用,可能与抑制NOS的活性有关。Objective：To study the protective effects and mechanism of nerve growth factor（NGF） on acute brain lesion induced by isehemia-reperfusion in rats.Method：The focal ischemia-reperfusion modal was made by thread embolism of middle cerebral artery,after treatment of NGF. The neurological grades were observed,the infarct size, the brain water content and the activity of nitric oxide synthase（NOS） were measured.Result：The neurological grades were significantly lowered after treatment compared with ischemia-reperfusion group （P〈0.05）,the infarct size,the brain water content and the activity of NOS in NGF treatment group were obviously lower than that in the ischemia-reperfusion group （P〈0,05）.Condusion：NGF can accelerate the nervous function recovery of the ischemiareperfusion injured rats,the mechanism is related to NGF prohibits NO production and play a role on amelioration of the brain ischemia-reperfusion iniury.

关 键 词：神经生长因子 脑缺血再灌注损伤 神经评分 一氧化氮合酶 大鼠 

分 类 号：R363[医药卫生—病理学]