GenBank数据库中鳜鱼微卫星位点的筛选及特征分析  

Isolation and characterization of microsatellite loci of Siniperca chuatsi from GenBank database

作　　者：匡刚桥[1] 刘臻[1] 鲁双庆[1] 刘红玉[2] 张建社[1] 肖调义[3] 

机构地区：[1]长沙大学生物工程与环境科学系,长沙410003 [2]湖南大学环境科学与工程系,长沙410082 [3]湖南农业大学动物科技学院,长沙410128

出　　处：《动物学报》2007年第1期184-189,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(30571414);湖南省自然科学基金(06JJ20056);湖南省教育厅项目(06C165)~~

摘　　要：This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats(SSRs),or microsatellites.A bioinformatic analysis of 304 nucleotide sequences in Siniperca chuatsi GenBank identified 22 sequences containing 30 microsatellites,account for 9.87% of wholly GenBank database.Cluster analysis indicated that 16 dinucleotide pairs were the most abundant microsatellites,accounting for 53.33% of the total microsatellite-containing sequences;11 trinucleotide repeats and 3 tetranucleotide repeats were found in these microsatellite sequences,accounting for 36.66% and 10.01% respective.17 primer pairs were designed from these microsatellite sequences.Among the 17 primer pairs,13 pairs have amplified products,6 pairs can achieve clear PCR products by Electrophoresis.Genotype analysis results indicated that:the average polymorphism information contents(PIC)were 0.692(ranged:0.567-0.806),were all polymorphic loci.This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats （SSRs）, or microsatellites. A bioinformatic analysis of 304 nucleotide sequences in Siniperca chuatsi GenBank identified 22 sequences containing 30 microsatellites, account for 9.87% of wholly GenBank database. Cluster analysis indicated that 16 dinucleotide pairs were the most abundant microsatellites, accounting for 53.33 % of the total microsatellite-containing sequences; 11 trinucleotide repeats and 3 tetranucleotide repeats were found in these microsatellite sequences, accounting for 36.66 % and 10.01% respective. 17 primer pairs were designed from these microsatellite sequences. Among the 17 primer pairs, 13 pairs have amplified products, 6 pairs can achieve clear PCR products by Electrophoresis. Genotype analysis results indicated that： the average polymorphism information contents （PIC） were 0.692.

关 键 词：鳜鱼 微卫星 GENBANK数据库 

分 类 号：Q953[生物学—动物学]