银杏外种皮对钉螺的杀灭机理  

Lethal effects of extracts of Ginkgo biloba Sarcotesta upon the snail Oncomelania hupensis

作　　者：陈盛霞[1] 吴亮[1] 杨小明[2] 姜旭淦[1] 李龙根[3] 张蓉仙[2] 夏磊[2] 邵世和[1] 

机构地区：[1]江苏大学医学技术学院,江苏镇江212013 [2]江苏大学化学化工学院,江苏镇江212013 [3]镇江市疾病预防控制中心,江苏镇江212003

出　　处：《动物学报》2007年第1期190-194,共5页ACTA ZOOLOGICA SINICA

基　　金：江苏省社会发展基金资助项目(BS2005042)~~

摘　　要：To study the toxicity of extracts of Ginkgo biloba sarcotesta to Oncomelania hupensis,snails were exposed to 40% and 80% of 24 h LC 50 of the extract of Ginkgo bilba for 24 h,choline esterase(ChE),alanine aminotransferase(ALT),alkaline phosphatase(ALP),lactate dehydrogenase(LDH),succino dehydrogenase(SDH),malic dehydrogenase(MDH)activities in cephalopodium and liver were determined by enzyme kinetic assay.Arecoline and niclosamide were used as reference molluscicides.The results showed that sarcotesta of Ginkgo biloba could inhibit ChE,ALT,ALP and MDH activities both in cephalopodium and liver;arecoline could inhibit ChE,ALP,SDH and MDH activities in cephalopodium and ChE,ALT,ALP,SDH and MDH activities in liver.Niclosamide had inhibitory effects upon ChE,ALT,ALP,SDH and MDH activities in cephalopodium,and ChE,ALT,ALP and SDH activities in liver.All three molluscicides did not inhibite LDH activity in cephalopodium and liver.These results indicate that lethal effects of extracts of sarcotesta of Ginkgo biloba are mediated via inhibition of MDH activitiy,and interference with the NADH respiratory chains.Inhibition of vital enzymic mechanisms causes snails to death.To study the toxicity of extracts of Ginkgo biloba sarcotesta to Oncomelania hupensis, snails were exposed to 40% and 80% of 24 h LC50 of the extract of Ginkgo bilba for 24 h, choline esterase （ChE）, alanine aminotransferase （ALT）, alkaline phosphatase （ ALP）, lactate dehydrogenase （ LDH）, succino dehydrogenase （ SDH）, malic dehydrogenase （ MDH ） activities in cephalopodium and liver were determined by enzyme kinetic assay. Arecoline and niclosamide were used as reference molluscicides. The results showed that sarcotesta of Ginkgo biloba could inhibit ChE, ALT, ALP and MDH activities both in cephalopodium and liver; arecoline could inhibit ChE, ALP, SDH and MDH activities in cephalopodium and ChE, ALT, ALP, SDH and MDH activities in liver. Niclosamide had inhibitory effects upon ChE, ALT, ALP, SDH and MDH activities in cephalopodium, and ChE, ALT, ALP and SDH activities in liver. All three molluscicides did not inhibite LDH activity in cephalopodium and liver. These results indicate that lethal effects of extracts of sarcotesta of Ginkgo biloba are mediated via inhibition of MDH activitiy, and interference with the NADH respiratory chains. Inhibition of vital enzymic mechanisms causes snails to death.

关 键 词：Oncomelania hupensis Enzyme kinetic assay Sarcotesta of Ginkgo biloba ARECOLINE NICLOSAMIDE 

分 类 号：S482.59[农业科学—农药学]