扣囊复膜孢酵母β-葡萄糖苷酶基因在工业酿酒酵母中的表达  

Expression of BGL Gene from Saccharomycopsis fibuligera in Industrial Saccharomyces cerevisiae

作　　者：周衍[1] 张梁[1] 王正祥[1] 石贵阳[1] 

机构地区：[1]江南大学生物工程学院生物资源研究室,无锡214036

出　　处：《中国生物工程杂志》2007年第2期64-69,共6页China Biotechnology

基　　金：国家"十五"科技攻关项目(2001BA501A01)

摘　　要：以工业酿酒酵母菌株(Saccharomyces cerevisiaeY)为研究对象,针对其复杂的生理生化遗传特性,建立了相对应的转化体系。以pRS41H质粒为基础载体,构建了含有工业酿酒酵母自身的gpd2启动子、终止子和扣囊复膜孢酵母的β-葡萄糖苷酶基因bgl的重组质粒pRS-gb。电击转化进入工业酿酒酵母细胞,潮霉素抗性筛选,获得重组菌。该重组菌可以在以纤维二糖为唯一碳源的培养基中生长,培养36h,β-葡萄糖苷酶酶活达到0.967μ/ml。以纤维二糖为唯一碳源的酒精发酵中,酒精度可以达到0.92g/l。这对工业生产中利用纤维素为原料发酵生产酒精具有重要意义。The industrial Saccharomyces cerevisiae Y was used as the host strain. According to complex physiological and biochemical genetic characteristics of industrial S. cerevisiae Y, a corresponding transforming system was built. Then a recombinant plasmid （pRS-gb） was constructed and successfully transformed into S. cerevisiae Y. The pRS-gb was based on shuttling expression vector pRS41H and contained the bgll gene from Saccharomycopsis fibuliger , gpd2 promotor and terminator genes from industrial S. cerevisiae Y. The recombined yeast （Saccharomyces cerevisiae BGL-19） could grow on the medium with cellobiose as sole carbon source and the highest enzyme activity （0. 967μ/ml） was achieved at 36h. The ethanol concentration reached 0.92g/l when cellobiose was used as sole carbon source, which was higher than using S. cerevisiae Y. It was important for ethanol production using cellulose in industrial application.

关 键 词：工业酿酒酵母 潮霉素B 电击转化 Β-葡萄糖苷酶 

分 类 号：TQ223.122[化学工程—有机化工] Q78[生物学—分子生物学]