新分离Microbacterium sp.XT11菌产黄原胶降解酶生产条件的优化研究  

Production of Xanthan-Degrading Enzyme by Newly Isolated Microbacterium sp. XT11

作　　者：韩秋惠[1] 李冲伟[1] 王梅 金朝霞[1] 李宪臻[1] 

机构地区：[1]大连轻工业学院生物与食品工程系,大连116034 [2]辽宁进出口商品检验检疫局理化检测中心,大连116001

出　　处：《生物技术通报》2006年第C00期469-471,475,共4页Biotechnology Bulletin

摘　　要：新分离Microbacteriumsp.XT11菌能够合成黄原胶降解酶,将植物病原菌野油菜黄单孢菌分泌的毒素因子黄原胶分解,生成具有激发子和抗微生物活性的黄原胶寡糖。实验确认,黄原胶和酵母浸粉分别是XT11菌生产黄原胶降解酶的最适碳源和氮源,获得最高酶活力的最低碳源和氮源浓度均为0.3%。XT11菌生产黄原胶降解酶的最适条件为:培养温度28℃,培养基起始pH7.0,转速150r/min。The xanthan-degrading enzyme produced by newly isolated Microbacterium sp. XT11 could fragment xanthan which is the pathogen of phytobacterium Xanthomonas campestris pv. campestris. The xantho-oligosaccharides produced from xanthan have elicitor-active and antimicrobial activity. It has been shown that xanthan and yeast extract are respectively the optimum for the production of xanthan-degrading enzyme by XT11 strain, the minimum concentrations of which all are 0. 3%. The optimal culture conditions for xanthan-degrading enzyme production by XT11 are that the XT11 is incubated at 28℃ and 150 rpm in the culture at pH7.0.

关 键 词：黄原胶降解酶 黄原胶 发酵 生产条件 MICROBACTERIUM 

分 类 号：Q814[生物学—生物工程]