琼脂扩散法测定乳链菌肽效价的优化  

Optimization of Agar Diffusion Bioassay for Nisin

作　　者：张国只[1] 陈林海[1] 杨天佑[1] 陈洪卫[1] 王利敏[1] 段宇珩[1] 秦广雍[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,河南郑州450052

出　　处：《食品科学》2007年第3期175-178,共4页Food Science

基　　金：国家重点基础发展计划(973计划)项目(2004CB719604)

摘　　要：对影响琼脂扩散法测定乳链菌肽效价的四个主要因素:琼脂浓度、Tween20、培养温度、预培养进行了研究。结果表明,0.75%琼脂的效价检测培养基、0.5% Tween20的添加以及30℃培养条件,可提高乳链菌肽效价测定的灵敏度和精确性;4℃预培养有利于乳链菌肽在琼脂中的扩散。The four major factors agar, Tween20, culture temperature and pre-diffusion, affecting the agar diffusion bioassay for nisin were stndied. The results showed that when agar concentration is 0.75%, the addition of Tween20 0.5%, and the culture temperature 30℃, the accuracy and sensitivity of agar bioassay for nisin can be improved. And the pre-diffusion at 4℃ can enhance the diffusion of nisin in agar.

关 键 词：乳链菌肽 琼脂扩散 预培养 

分 类 号：Q331[生物学—遗传学]