Genistein对涎腺腺样囊性癌细胞生长及Survivin表达的影响  

Effects of Genistein on the Proliferation and Expression of Survivin in Salivary Adenoid Cystic Carcinoma Cell Line SACC-83

作　　者：马杰[1] 宗志红[2] 王兆元[3] 钟鸣[3] 

机构地区：[1]中国医科大学第二临床医院口腔科,辽宁沈阳110004 [2]中国医科大学生化教研室 [3]中国医科大学口腔医院病理科,辽宁沈阳110001

出　　处：《华西口腔医学杂志》2007年第1期97-99,共3页West China Journal of Stomatology

基　　金：辽宁省教育厅科学研究计划资助项目(05L533)

摘　　要：目的研究酪氨酸蛋白激酶抑制剂Genistein对人涎腺腺样囊性癌细胞株SACC-83细胞增殖及Survivin表达的影响。方法以不同浓度Genistein作用于SACC-83细胞不同时间后,用MTT法分析细胞的生长增殖,用流式细胞术检测细胞凋亡,用Western印迹分析及电泳凝胶成像分析软件定量检测Survivin的表达。结果Genistein对SACC-83细胞有一定的抗增殖作用,且当其作用到一定时间达到一定的浓度后,该作用与浓度及时间呈依赖关系;SACC-83细胞经220μmol/L Genistein作用3 d其生长明显受到抑制,并显著诱导细胞凋亡(P<0.01),同时伴有抗凋亡蛋白Survivin的表达下调。结论Genistein能明显抑制人涎腺腺样囊性癌细胞株SACC-83细胞生长并诱导凋亡;Survivin的表达减少可能是Genistein诱导凋亡的机制之一。Objective To investigate the anti-proliferation effect of tyrosine protein kinase inhibitor, Genistein, on human salivary adenoid cystic carcinoma cell line SACC-83, and its effect on Survivin expression. Methods SACC-83 cells were treated with different concentration Genistein for different time, cell survival rate was calculated with MTT assay, apoptosis wasdetected with flow cytometry, the expression of Survivin was quantitatively analyzed by Western blotting and FluorChem V2.0 software. Results When treated with C, enistein of certain concentration for certain time, SACC-83 cell growth was significantly inhibited. With the increase of concentration and elongation of acting time, the inhibitory effects increase. Treated with 220 μmol/L Genistein for 72 hours, SACC-83 cell growth was significantly inhibited, cell apoptosis was induced（P〈0.01）, and the expression of Survivin decreased. Conclusion Genistein inhibits the growth of human salivary adenoid cystic carcinoma cell line SACC-83, and induces cell apoptosis; the decrease of Survivin expression may be one of the mechanisms of Genistein inducing apoptosis.

关 键 词：GENISTEIN 涎腺腺样囊性癌 SURVIVIN 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]