CO_2对人卵巢癌细胞SKOV3转移因子表达的影响  

Effects of carbon dioxide on the expression of metastatic factors in human ovarian carcinoma cell line SKOV3

作　　者：黄金[1] 唐均英[1] 姚珍薇[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《现代妇产科进展》2007年第1期29-32,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨二氧化碳(CO_2)对卵巢恶性肿瘤细胞转移能力的影响。方法:体外培养人卵巢浆液性乳头状腺癌SKOV3细胞株,分为CO_2组、N_2组和对照组,分别在CO_2及N_2处理后2h,置于常规条件下培养12、24、48h通过RT-PCR和Western Blot检测不同时相血管内皮生长因子C(VEGF-C)和基质金属蛋白酶9(MMP9)的表达。结果:CO_2处理后,SKOV3细胞VEGF-C和MMP9的mRNA和蛋白质表达显著增加(P＜0．05);N_2处理后,SKOV3细胞VEGF-C和MMP9的mRNA和蛋白质表达较对照组增加(P＜0．05)。结论:CO_2促进了SKOV3 VEGF-C和MMP9的表达,对SKOV3的转移能力有增强作用。Objective ：To study the effects of carbon dioxide on the expression of metastatic factors in ovarian carcinoma cell line SKOV3. Method：Ovarian carcinoma cell line SKOV3 was treated with 100% carbon dioxide for 2 hours,then put into normal circumstance for 12hours,24hours,48hours,respectively;Examine the expressions of different times of VEGF-C and MMP9 by RT-PCR and Western Blot. Result： After the treatment of carbon dioxide, the expression of VEGF-C and MMP9 significantly increased compared with controlled groups. Conclusion：Carbon dioxide promotes the expression of VEGF-C and MMP9 in SKOV3, so it may improve the metastatic capability of ovarian carcinoma cell line.

关 键 词：卵巢肿瘤 二氧化碳 细胞株SKOV3 血管内皮生长因子-C 基质金属蛋白酶-9 

分 类 号：R737.3[医药卫生—肿瘤]