轮梗霉高产花生四烯酸的诱变育种研究  

Study on Arachidonic Acid Mutation Strains Breeding of Diasporangium sp.

作　　者：王安琪[1] 戴传超[1] 赵沫[1] 赵庆新[2] 周雷磊[1] 

机构地区：[1]南京师范大学生命科学学院微生物工程重点实验室,江苏南京210097 [2]盐城师范学院生命科学与技术学院,江苏盐城224002

出　　处：《食品科学》2007年第2期158-162,共5页Food Science

基　　金：江苏省教育厅自然科学基金项目(03KJB180065);江苏省高技术资助项目(BG2005326)

摘　　要：利用紫外线作为诱变剂,分别通过15℃的低温和0.09mg/ml乙酰水杨酸的筛选作用,筛选到两株花生四烯酸产量有较大提高的菌株,分别为LT1.12菌株(花生四烯酸产量提高46.41%)和A1.13菌株(花生四烯酸产量提高53.15%)。通过对脂肪酸组分的分析,确定乙酰水杨酸为更加具有针对性的筛选条件。并对用乙酰水杨酸筛选到的A1.13菌株进行了传代稳定性实验,证实A1.13菌株花生四烯酸产量提高这一性状是可以稳定遗传的。Using Diasporangium sp. as original strain, with low temperature 15℃ and 0.09 mg/ml aspirin as inhibitors, after UV radiation, some mutation strains were selected. Fermentation experiments assayed various strains in the biomass, arachidonic acid yield and fatty acids. The changes of fatty acids showed that the aspirin is the most effective inhibitor, and A1.13 strain is selected with its arachidonic acid yield enhanced 53.15%.

关 键 词：花生四烯酸 轮梗霉 诱变育种 

分 类 号：TQ921[轻工技术与工程—发酵工程]