保健食品建泽泻及其混淆品的PCR-RFLP分子鉴别  

Molecular Authentication of Alisma orientali (Sam.) Juzep Food from Its Adulterants by PCR-RFLP

作　　者：褚必海[1] 丁小余[1] 李雪霞[1] 贺佳[1] 丁鸽[1] 刘冬扬[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097

出　　处：《食品科学》2007年第2期177-181,共5页Food Science

基　　金：江苏省自然科学基金资助项目(BK2003101)

摘　　要：目的:寻找简易可重复的分子标记方法对保健食品建泽泻及其混淆品进行鉴定。方法:对建泽泻及其混淆品的rDNA ITS区进行PCR扩增、测序,运用ClustalX、Mega3.0、DNAMAN 4.0等软件对ITS区进行序列分析和PCR限制性酶切图谱分析(PCR-RFLP)。结果:依据建泽泻及其混品完整的rDNA ITS区片段长度,将慈菇和甘薯两种混淆品首先区分开;再通过PCR-RFLP分析,可将建泽泻从川泽泻、窄叶泽泻、小泽泻和芋等混淆品中成功鉴别出来。结论:rDNA ITS片段长度不足以用于鉴别保健食品建泽泻原料的真伪;PCR-RFLP可对保健食品建泽泻及其混淆品进行简便而快捷的分子鉴别。Aim： To develop a convenient and reproducible molecular marker method to identify the hygienical food of Alisma orientali from its adulterants. Method： PCR products of ITS regions were sequenced and analysed with Clustal X, Mega3.0 and restriction pattern analysis was performed with DNAMAN 4.0. Result： According to the length of rDNA ITS regions, Sagittaria sagittifolia Lima and Ipomoea batatas （L.） Lain were seperated first： further authentication of Alisma orientali from Alisma plantago-aquatica Lima, Alisma canaliculatum A.Braun et Bouche, Alisma nahum D.F.Cui and Colocasia esculenta （L.） Schott can be identifded sucessfuUy by PCR-RFLP （polymerase chain reaction-restriction fragment length polymorphism）. Conclusion： The length of rDNA ITS （intergenic spacer） regions cannot adequately distinguish hygienical food of Alisma orientali from its adulterants. PCR-RFLP is a simple and rapid method to authenticate the hygienical food ofAlisma orientali from its adulterants.

关 键 词：建泽泻 保健食品 混淆品 PCR-RFLP 分子鉴别 

分 类 号：Q93[生物学—微生物学]