普鲁士兰法测定胡杨中植物多酚含量  

A Study on the Determination of Phenolic Compounds Content in Populus euphratica with Prussian Blue spectrophotometry

作　　者：刘松[1] 李俊清[1] 廖蓉苏[1] 

机构地区：[1]北京林业大学

出　　处：《林业科技开发》2007年第2期42-44,共3页China Forestry Science and Technology

基　　金：国家自然科学基金项目"极端干旱环境下植物多酚防御机制基础研究"(编号:30471369)

摘　　要：首次采用普鲁士兰法对胡杨中植物多酚含量进行了研究。取胡杨枝粉碎后过40目筛,用丙酮水溶液提取液在45℃下浸提,提取液处理后用P-B法进行测定。首先从稳定性、最大吸收波长、线性关系、重复性和准确性等角度对P-B法进行了方法改进和验证。然后用改进的P-B法对胡杨枝提取液进行测定,测得结果是胡杨枝中总酚含量平均为1.191%、RSD为2.840%。Phenolic compounds content of Populus euphratica was assaged with Prussian Blue spectrophotometry, Populus euphratica branch were extracted at 45% with aqueous acetone and assaged with P-B method. The method was improved and verified in the aspects of stability,the largest absorb wavelength,linearity, precision and accuracy. Phenolic compounds average content in Populus euphratica was determinated at 0. 119 1% ,and RSD was 2. 840%.

关 键 词：胡杨 普鲁士兰分光光度法 总酚含量 

分 类 号：TS262.5[轻工技术与工程—发酵工程]