培养条件对枯草芽胞杆菌TU100生长和产生抗菌物质的影响  

The effect of culture conditions on growth and antibiotic production of Bacillus subtilis TU100

作　　者：赵瑞[1] 江木兰[2] 胡小加[2] 姚小飞[3] 

机构地区：[1]中国农业科学院研究生院,北京100062 [2]中国农业科学院油料作物研究所 [3]武汉生物工程学院生物工程系,湖北武汉431415

出　　处：《中国油料作物学报》2007年第1期69-73,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家"十五"科技攻关(2004BA520A16-030);湖北省科技攻关(2002AA201C02)

摘　　要：以在油菜根际高效定殖的枯草芽孢杆菌(Bacillus subtilis)TU100为材料,研究其主要抗菌物质的代谢条件。证明其主要拮抗物质属胞外分泌型,释放到培养液中,对油菜菌核病菌(Sclerotinia sclerotiorum)具有较高的抑制活性。TU100在28-30℃生长旺盛,产抗菌物质也较多;培养48h数量达最大值,培养72h抗菌物质的产生量达高峰。培养液的初始pH值6-8有利于TU100增殖,抗菌物质活性也最强;通气良好的条件下TU100菌株生长好、产抗菌物质量多;培养基C/N比对菌株生长也有重要影响,C/N比为15时有利于TU100生长以及抗菌物质的积累。The Bacillus subtilis TU100 isolated from the root surface of rape plant could secret large amount of extracellular antibiotic substance and has a strong inhibitory activity against Sclerotinia sclerotiorum. The strain produced more antibiotic substance at 30℃. The optimum initial pH of liquid culture broth for bacteria growth and antibiotic substance production was 6 - 8. Sufficient oxygen and high carbon - nitrogen ratio of liquid culture broth enhanced the bacteria growth and antibiotic substance production. The growth of bacteria reached the highest level at 48h, and the antibiotic substance production peaked at 72h.

关 键 词：枯草芽孢杆菌 抗菌物质 培养条件 C/N比 

分 类 号：S476[农业科学—农业昆虫与害虫防治]