培养条件对甲醇毕赤酵母异源表达木质素过氧化物酶的影响  

Effect of Cultivation Condition on Heterologous Expression of Lignin Peroxidase in Pichia methanolica

作　　者：王海宽[1] 路福平[1] 

机构地区：[1]天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2007年第1期33-36,共4页Journal of Tianjin University of Science & Technology

摘　　要：对含有黄孢原毛平革菌木质素过氧化物酶基因(LipH8)的甲醇毕赤酵母培养基和发酵方法进行了研究．摇瓶培养条件研究结果表明:用11°Bx麦芽汁培养工程菌,然后用高温浸提液置换诱导产酶较好．5 L发酵罐产酶放大实验结果表明,木质素过氧化物酶较稳定,在72 h木质素过氧化物酶酶活达到最高7 568 U/L．The medium and fermentation method of Pichia methanolica with lignin peroxidase gene （LipH8） of Phaneroehaete chrysosporium was investigated. The optimum fermentation method of lignin peroxidase was studied by shake-flask culture. It is good that Pichia methanolica was cultured in the 11°Bx wort medium and then induced in the high temperature malt extract medium. In 5 L fermenter the lignin peroxidase activity was stable and reached the highest activity of 7568 U/L.

关 键 词：异源表达 木质素过氧化物酶 甲醇毕赤酵母 黄孢原毛平革菌 

分 类 号：TQ92[轻工技术与工程—发酵工程]