胃癌中p16基因缺失与突变的研究  

Analysis of p16 Gene Deletion and Mutation in Gastric Carcinoma

作　　者：李进[1] 刘大仁[2] 熊炳贤[3] 聂鸿平[1] 

机构地区：[1]南昌大学研究生院医学部,2004级南昌330006 [2]南昌大学医学院学报编辑部,南昌330006 [3]南昌大学第二附属医院普外科,南昌330006

出　　处：《江西医学院学报》2007年第1期26-28,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的探讨胃癌组织中p16基因缺失与突变发生率及其临床意义。方法PCR方法扩增p16基因外显子1(E1)及外显子2(E2)、检测等位基因纯合子缺失;紫外分光光度计检测DNA纯度浓度;应用DNA测序方法分析基因突变情况。结果30例胃癌组织中E1、E2纯合性缺失分别为6例(20.0%)及3例(10.0%),9例标本中6例属于Ⅲ期低分化癌;所有标本未检出点突变。结论p16基因缺失是胃癌发生发展中一重要事件,对胃癌的诊断和治疗有重要意义。Objective To investigate the frequencies of homozygous deletion and P16 gene and to evaluate the clinic significance. Methods PCR technique was used to detect homozygous deletions of exon 1 and exon 2 of p16 gene and DNA sequencing technique was used to detect the genetic mutation. Results The homozygous deletion ratio of exon 1 and exon 2 of p16 gene in 30 gastric cancer tissues was 6 out of 30 cases and 3 out of 30 cases,respectively. Six of which belonged to stage Ⅲ, poorly differentiated cancer. In DNA sequencing, no mutation was found in all cases. Conclusion The abnormality of p16 gene is an important event in the progres sion of gastric cancer and may be associated with the development of gastric cancer.

关 键 词：基因突变 基因缺失 胃肿瘤 P16基因 

分 类 号：Q754[生物学—分子生物学] R735.2[医药卫生—肿瘤]