胃癌微卫星不稳定性的临床研究  

Clinical research of microsatellite instability in gastric carcinoma

作　　者：孙晓卫[1] 詹友庆[1] 李威[1] 徐立[1] 李锦添[1] 陈小君[1] 张如华[1] 徐大志[1] 

机构地区：[1]中山大学肿瘤防治中心,华南肿瘤学国家重点实验室,广东广州510060

出　　处：《中华肿瘤防治杂志》2007年第2期118-121,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的检测微卫星不稳定性在胃癌中的发生情况,探讨其与临床病理参数的关系。方法选取D2S123和D3S1298两个微卫星位点,采用PCR-银染法,对36例胃癌标本进行检测;胃癌微卫星不稳定性与临床病理参数的关系采用χ2检验或费歇尔精确检验。结果两个位点共检出MSI阳性11例,发生率为30.6%(11/36);与胃癌微卫星不稳定性有关的临床病理因素为肿瘤的组织学类型(P=0.048)及有无淋巴结转移(P=0.034)。结论微卫星不稳定性在胃癌的发生及发展中可能起重要作用,是正常细胞癌变的可能途径之一;中-高分化腺癌或有淋巴结转移的胃癌患者微卫星不稳定的发生率高。OBJECTIVE： To detect the incidence of microsatellite instability （MSI） in gastric carcinoma and investigate the correlation between MIS and clinico-pathological features. METHODS： Two microsatellite loci D2S123, D3S1298 were analyzed in 36 samples by polymerase chain reaction （PCR）-silver staining methods. Chi-square tests or Fisher＇s exact test was used for statistical analysis. RESULTS.. Eleven of 36 cases were categorized as positive MSI in two microsatellite markers, and the total incidence of MSI was 30. 6%. Histological type （P= 0.048） and lymph node metastasis （P=0. 034） were associated with MSI. CONCLUSION： MSI may play a significant role in genesis and development of gastric carcinoma, and may be one of paths for normal cell canceration. Moderatedly-well differentiated adenocarcinoma or lymph node metastasis-positive patients with gastric carcinoma easily demonstrate microsatellite instability.

关 键 词：胃肿瘤/病理学 DNA 卫星 基因组不稳定性 胃肿瘤/遗传学 

分 类 号：R735.2[医药卫生—肿瘤]