一株降解PCBs嗜盐菌的筛选及产酶条件研究  

Screening of a PCBs-degrading Halophilic Bacterium and Its Enzyme-producing Conditions

作　　者：赵喆[1] 张兰英[1] 李爽[1] 刘鹏[1] 

机构地区：[1]吉林大学环境与资源学院,吉林长春130026

出　　处：《农业环境科学学报》2007年第1期310-313,共4页Journal of Agro-Environment Science

基　　金：国家重点基础研究发展规划项目资助(2004CB4185)

摘　　要：从深海底泥中提取出一株生长盐度在15%的降解PCBs的嗜盐菌,并将其命名为B2.6。此菌株能以PCBs为惟一碳源和能源生长,72h的降解率可达90%以上。对其进行了形态观察、革兰氏染色及各项生理生化指标鉴定,并和Biolog细菌鉴定系统鉴定的结果进行了比对。研究结果表明,菌株B2.6合成PCBs降解酶的模式为延续合成型;主族的一价离子Na+、K+能够提高PCBs降解酶的酶活,过渡金属离子Co2+、Mn2+、Cu2+、Fe2+和Fe3+对酶活则有一定程度的抑制;此酶的最适温度和pH值分别为25～35℃和pH6～8。Polychlorinated biphenyls （PCBs） are persistent organic pollutants （POPs）. This experiment screened a halophilic bacterium from the deep sea sediments, which could grow at the salinity of 15%, and designated as B2.6. The cells of this strain were rod shaped, Gram negative, motile, could grow in pH 5.0-9.0 and 10-37 ℃ This bacterium could make growth using PCBs as the sole source of carbon and energy. The experiment indicated that at 30 ℃, pH 7 and 8, the inoculation amount 5 mL, the concentration of PCBs below 3mg·L^-1, the degradation ratio of PCBs could reach 90% at 72 h. The traditional morphology, physiological and biochemical properties as well as identified system of Biolog were applied to the bacteria classification. The pattern of PCBs-degrading enzyme production of strain B2.6 was continuous synthesis. The activity of PCBs-degrading enzyme could be enhanced by the ions of I A, such as Na^＋ and K^＋, while it might be inhibited by the transitional metal ions, such as Co^2＋, Mn^2＋, Cu^2＋, Fe^2＋ and Fe^3＋ in a certain degree. The optimal temperature, pH value and concentration of NaCI for the PCBs-degrading enzyme were 25-35 ℃, pH 6-8 and 50 g·L^-1, respectively.

关 键 词：嗜盐菌 多氯联苯 生物降解 BIOLOG 

分 类 号：X172[环境科学与工程—环境科学]