Determination of vinyl orientation of mouse neuroglobin by 2D nuclear Overhauser spectroscopy  

作　　者：Su Yuan Zhou Xiao Yan Wei 

机构地区：[1]Department of Biomechanlcs, Shanghai University of Sports, Shanghai 200438, China

出　　处：《Chinese Chemical Letters》2007年第3期319-321,共3页中国化学快报（英文版）

基　　金：This research was supported by the grants from the National Natural Science Foundation of China(No.10572097);Shanghai Natural Science Foundation(No.05ZR14111);Shanghai leading Academic Discipline Project(No.T0901).

摘　　要：^1H NMR has been used to determine the 2-, 4-vinyl orientation of heme active site from oxidized mouse neuroglobin （mNgb）. The NOEs between 3-methyl and Hα, Hβ of 2-vinyl, together with the NOEs between 5-methyl and Hα, Hβ of 4-vinyl, allowed the unambiguous determination of trans and cis orientations for the 2- and 4-vinyl groups in the mNgb, respectively.^1H NMR has been used to determine the 2-, 4-vinyl orientation of heme active site from oxidized mouse neuroglobin （mNgb）. The NOEs between 3-methyl and Hα, Hβ of 2-vinyl, together with the NOEs between 5-methyl and Hα, Hβ of 4-vinyl, allowed the unambiguous determination of trans and cis orientations for the 2- and 4-vinyl groups in the mNgb, respectively.

关 键 词：HEME Vinyl orientation NEUROGLOBIN 

分 类 号：Q513.4[生物学—生物化学]