Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226  

作　　者：HAO ZHANG NA LI YUE CHEN LING-YUN HUANG YI-CHING WANG GANG FANG DA-CHENG HE XUE-YUAN XIAO 

机构地区：[1]Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Universities＇ Confederated Institute of Proteomics, Beijing Normal University, Beijing 100875, China [2]Department of Life Sciences, National Taiwan Normal Universities, Sec. 4 Tingchou Rd, Taipei 116, Taiwan

出　　处：《Biomedical and Environmental Sciences》2007年第1期24-32,共9页生物医学与环境科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China (Grant No.30370712);Beijing Key Project (Grant No. 7051002).

摘　　要：Objective To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer. Method Proteomic technique was used to analyze human lung squamous carcinoma cell line NCI-H226. The proteins of the NCI-H226 cells were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Results The results showed that a good reproducibility of the 2-D gel pattern was attained. The position deviation of matched spots among three 2-D gels was 1.95±0.53 mm in the isoelectric focusing direction, and 1.73±0.45 mm in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis direction. One hundred and twenty-seven proteins, including enzymes, signal transduction proteins, structure proteins, transport proteins, etc. were characterized, of which, 29 identified proteins in NCI-H226 cells were reported for the first time to be involved in lung cancer carcinogenesis. Conclusion The information obtained from this study could provide some valuable clues for further study on the carcinogenetic mechanism of different types of lung cancer, and may help us to discover some potential subtype-specific biomarkers of lung cancer.

关 键 词：Lung squamous carcinoma NCI-H226 cell line PROTEOMICS Two-dimensional datapase 

分 类 号：R734.2[医药卫生—肿瘤]