DC-SIGNR基因绞链区重复序列多态性检测方法的建立与应用  

Determination of DC-SIGN and DC-SIGNR repeat region variations

作　　者：王辉[1] Huanliang Liu 王春慧[2] 李丽雄[1] 胡亚冬[3] 童新灯[4] 晏路标[3] 周平[3] 施玲玲[3] 肖昕[3] 周伯平[1] 朱托夫[2] 

机构地区：[1]深圳市东湖医院,广东深圳518020 [2]美国华盛顿大学医学院 [3]暨南大学医学院艾滋病基因与疫苗研究开发中心,广东广州510080 [4]深圳市东湖医院广东,深圳518020

出　　处：《中国艾滋病性病》2007年第1期39-41,共3页Chinese Journal of Aids & STD

基　　金：美国华盛顿大学CFAR基金资助[编号P30AI-27757-18(S3)];深圳市2005科技计划重点项目资助(编号JH200505270384A)

摘　　要：目的建立树突状细胞表面的蛋白质DC-SIGNR基因绞链区重复序列多态性的检测方法,了解宿主因素在抗艾滋病病毒1型(HIV-1)所起的作用。方法设计特定引物,用PCR方法对DC-SIGNR基因绞链区重复序列进行扩增,用凝胶电泳法对其产物进行分析。结果根据DC-SIGNR绞链区重复序列的数量,DC-SIGNR绞链区重复序列具有高度基因多态性,而DC-SIGN则罕见基因多态性。结论所建立的检测DC-SIGNR绞链区重复序列的方法简单,易于掌握。由于DC-SIGNR重复序列数量的改变可能会影响其正常功能,从而影响宿主与HIV-1的结合能力,因此该法为研究宿主因素在抗HIV-1感染中所起作用的一种好方法。Objective To establish method to detect DC-SIGN and DC-SIGNR repeat region variations for its use in studying Chinese Han population. Methods Specific primers for amplifying DC-SIGNR repeat regions were designed,and the PCR products were analyzed by electrophoresis. Results Based on the number of tandem repeats,DC-SIGNR was highly polymorphic in the repeat region, while variations in DC-SIGN repeat region were rare. Conclusion Since the change in the number of DC-SIGNR repeats may influence their normal functions and their binding capacity to HIV- 1 ,the determination of the repeats is a simple and good method to be used to study the role of host factors in HIV-1 infection.

关 键 词：DC-SIGN DC-SIGNR 等位基因 基因型 艾滋病病毒 

分 类 号：R373.9[医药卫生—病原生物学]