Kruppel样因子4过表达对C_2C_(12)肌原细胞中热休克蛋白25表达的影响  

Effects of the Overexpression of Kruppel-Like Factor 4 on the Expression of Heat Shock Protein25 in C_2C_(12) Cell

作　　者：王婧[1] 刘瑛[1] 易宇欣[1] 张华莉[1] 刘梅冬[1] 肖献忠[1] 邓恭华[1] 

机构地区：[1]中南大学湘雅医学院病理生理学教研室

出　　处：《中国动脉硬化杂志》2006年第10期841-844,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30400169;30571746;30571846);国家973重点项目(G2000056900)资助

摘　　要：目的观察Kruppel样因子4过表达对热休克蛋白25表达的影响。方法运用稳定转染pcDNA3.1/myc-His(-)和Kruppel样因子4-pcDNA3.1/myc-His(-)两种细胞株,采用逆转录聚合酶链反应观察Kruppel样因子4在生理状态和热休克反应时对热休克蛋白25 mRNA表达的影响;采用Western blot观察Kruppel样因子4在生理状态和热休克反应时对热休克蛋白25蛋白表达的影响。结果在正常状态下,两种细胞热休克蛋白25 mRNA及蛋白表达水平均较低。生理状态下,热休克恢复1 h及3 h Kruppel样因子4过表达细胞株热休克蛋白25 mRNA表达水平明显高于转空载体细胞;至热休克恢复6 h后两组细胞热休克蛋白25 mRNA表达无明显差异。在生理状态及热休克恢复不同时间后,Kruppel样因子4过表达细胞热休克蛋白25蛋白表达水平均较空载体组明显增加。结论在生理状态下和热休克反应中,Kruppel样因子4过表达均能够促进C2C12肌原细胞中热休克蛋白25高表达。Aim To investigate the effects of Kruppel-like factor 4（KLF4）. overexpression on the expression of heat shock protein25 （HSP25）. Methods Two stable cell lines were made by transforming pcDNA3, 1/Myc-His （-） or pcDNA3. 1/Myc-His-KLF4 into C2 C12 cell. Reverse transcription polyrnerase chain reaefion（RT-PCR） and Western Blot were employed to study the influence of KLF4 on the expression of HSP25 mRNA and protein respectively under normal physiological and heat shock conditions. Results Under normal physiological condition, the expression of HSP25 mRNA and protein in these two cell lines are relatively low. However, HSP25 mRNA level in KLF4 over expressed cell was significantly higher than that of empty vector cell lines not only under normal conditions but when recovered one hour and three hours after heat shock. Six hours after heat shock, the levels of HSP25 mRNA showed no significant difference between these two cell lines. Under both normal physio- logical conditions and different time intervals during the recovery of heat shock, the HSP25 protein level was higher in KLF4 over expressed cell line than that in the vector line. Collelusions Under both physiological and heat shock conditions, KLF4 over expression can enhance the expression of HSP25 in the myogenic C2C12 cell.

关 键 词：病理学与病理生理学 Kruppel样因子4 热休克蛋白25 稳定转染 C2C12肌原细胞 过表达 空载体 

分 类 号：R363[医药卫生—病理学]