运动发酵单胞耐酸菌株的诱变筛选及发酵条件的优化  

Selection and optimization of culture condition of acid-resistant mutant Zymomonas mobilis

作　　者：傅云霞[1] 蔺玉萍[1] 张木清[1] 陈柏铨[2] 余伟俊[2] 陈如凯[1] 许燕[1] 郭春芳[1] 

机构地区：[1]福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350002 [2]中国科学院广州能源研究所,广东广州510070

出　　处：《福建农林大学学报（自然科学版）》2007年第1期96-101,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金资助项目(2006J0003);国家攻关先导项目(2002BA544C)

摘　　要：通过对运动发酵单胞菌原始菌株(ZM6)的耐酸驯化、紫外诱变、小型初筛和复筛,获得一株耐酸突变菌株ZM6-3-2.通过突变菌株与出发菌株以及酵母发酵性能的比较表明,ZM6-3-2的发酵蔗汁性能显著优于ZM6菌株和工业生产乙醇的酵母,其最终乙醇质量浓度为56.64 g.L-1,比ZM6菌株的52.18 g.L-1提高了4.46 g.L-1,而且最终乙醇质量浓度、总糖出酒率、总糖利用率均比酵母高出约10%.通过正交试验对该耐酸菌株的发酵培养基进行优化并进行验证,发现含250 g.L-1总糖、10 g.L-1酵母膏、2 g.L-1KH2PO4、0.5 g.L-1(NH4)2SO4、1 g.L-1MgSO4.7H2O,pH 5.5的培养基最有利于ZM6-3-2的发酵,发酵时间短,产乙醇多.Acid-rosistant mutant strain ZM6-3-2 of Zymomonas mobilis was selected from the original strain ofZ. mobilis ZM6 via domestication of acid-resistance and mutation induced by UV, as well as primary and secondary screening in shake flask scale. Compared with strain ZM6 and industrial yeast in sugarcane juice media, mutant strain ZM6-3-2 produced ethanol of 56.64 g·L^-1 , 4.46 g·L^-1 higher than that fermented with strain ZM6. It also utilized 10% more sugar for fermentation than those of yeast. Based on the orthogonal experiment, the optimum fermentation medium of ZM6-3-2 was as follows： 250g·L^-1 total sugar, 10g·L^-1 yeast extraction, 2g·L^-1 KH2PO4, 0.5 g·L^-1 （NH4）2SO4, 1 g·L^-1 MgSO4 ·7H2O and pH 5.5.

关 键 词：运动发酵单胞菌 耐酸驯化 诱变 培养条件 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]