微生物发酵法中试生产1,3-丙二醇  

Production of 1,3-propanediol by microbiological fermentation on a pilot scale

作　　者：刘海军[1] 张代佳[1] 徐友海[1] 孙亚琴[1] 吕继萍[2] 牟英[1] 修志龙[1] 

机构地区：[1]大连理工大学生物科学与工程系,辽宁大连116024 [2]中国石油吉林石化分公司研究院,吉林吉林132021

出　　处：《现代化工》2007年第2期56-58,60,共4页Modern Chemical Industry

基　　金：国家'十五'攻关项目(2004BA713B06-03);国家'973'项目(2003CB716000)

摘　　要：以甘油为原料,利用克雷伯氏菌进行了发酵罐容积规模分别为1 m3和20 m3的发酵法1,3-丙二醇生产的中试研究。试验结果表明:1m3和20m3的发酵罐最终发酵液中1,3-丙二醇平均质量浓度分别达到了74.6 g/L和63.9 g/L,1,3-丙二醇相对于甘油的得率分别为61.2%和49.9%。采用发酵液醇沉预处理、再精馏的专利技术可分离得到纯度大于99%的1,3-丙二醇产品,分离收率大于85%,产品质量达到了聚对苯二甲酸丙二酯(PTT)聚合反应的要求。成本分析表明,生物法生产1,3-丙二醇技术是经济可行的。Fermentative production of 1,3-propanediol from glycerol by KlebsieUa pneumoniae has been conducted up to the scale of 1 m^3 and 20 m^3 .The experimental results showed that the final mass concentration,molar yield of 1,3-propanediol to glycerol were 74.6 g/L and 61.2% in 1 m^3 reactor, 63.9 g/L and 49.9% in 20 m^3 reactor, respectively. By using ethanol precipitative pretreatrnent and rectification patent technology, the product 1,3-propanediol with purity of 99% had been harvested, the seperation yield of 1,3-propanediol was greater than 85 %, and the product quality had met the polymerization standard. The cost analysis demonstrated that the microbial production of 1,3-propanediol was economically feasible.

关 键 词：1 3-丙二醇 克雷伯氏菌 甘油 微生物发酵 中试 

分 类 号：TQ923[轻工技术与工程—发酵工程]