CD44拼接变异体V6与上皮钙粘附素在胰腺癌中的表达及意义  

Significance and expression of CD44 Splice varint V6 and E-cadherin in pancreatic carcinomas

作　　者：介建政[1] 田力[2] 王巍[2] 刘治滨[1] 王文跃[1] 

机构地区：[1]卫生部中日友好医院,北京100029 [2]吉林大学中日联谊医院基本外科,吉林长春130031

出　　处：《中国实验诊断学》2007年第3期309-311,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨CD44拼接变异体V6(CD44 Splice varint V6,CD44V6)和上皮钙粘附素(E-Cadherin,E-CD)在胰腺癌中表达及与胰腺癌浸润、转移的关系,评价CD44V6和E-CD在胰腺癌浸润转移中的作用。方法采用SP免疫组化染色方法,检测43例胰腺癌组织中CD44V6和E-CD的表达情况。结果正常胰腺导管上皮细胞CD44V6呈阴性表达,胰腺癌组织中CD44V6阳性表达率达58.1%(25/43),CD44V6阳性表达与胰腺癌浸润深度、淋巴结转移情况密切相关(P<0.05),与胰腺癌组织分化程度无关(P>0.05)。正常胰腺导管上皮细胞E-CD呈强阳性表达,60.5%(26/43)的胰腺癌组织中E-CD呈异常表达,E-CD的异常表达与胰腺癌组织分化程度、浸润程度、淋巴结转移情况关系密切(P<0.01)。CD44V6和E-CD在胰腺癌中的表达无明显相关性(P>0.05)。结论CD44V6阳性表达和E-CD异常表达与胰腺癌浸润、转移关系密切,检测CD44V6和E-CD表达有助于对胰腺癌浸润转移潜能的判断,二者可以作为胰腺癌浸润转移的生物学指标。Objective To elucidate the expression of CD44V6 and F-CD in invasion and metastasis of pancreatic cancer,and evaluate its value for the invasion and tumor metastatic potential.Methods The expression of CD44V6 and E-CD was detected in 43 eases of pancreatic cancer. Streptavidin-peroxidase immunohistochemistry. Results The normal pancreatic tissue did not express CD44V6 protein, whereas the positive rate of CD44V6 expression was 58.1% （25/43） in pancreatic cancer. The positive expression of CD44V6 in the pancreatic cancer was significantly correlated with the depth of invasion and lymph node metastasis （ P 〈 0.05） and it was not associated with the differentiation （ P 〉 0.05 ）. The normal pancreatic tissue strongly expressed E- CD, whereas 60.5 % （26/ 43） pancreatic cancers showed abnormal expression of E-CD . The significant correlation was observed between abnormal expression and differentiation, the depth of invasion and the lymph node metastasis （ P 〈 0.05）. There was no significant association with the expression of CD44V6 and E-CD （ P 〉 0,05）. CondusIon The positive expression of CD44V6 and the abnormal expression of E-CD has a close relation with the invasion,metastasis of panereafe cancer. The determinafon of CD44V6 and E-CD will help in predicting tumor invasion and metastatic potential. CD44V6 and E-CD can serve as the biological marker of invasion and metastasis of pancreatic cancer.

关 键 词：胰腺癌 CD44V6 E-CD 浸润转移 

分 类 号：R735.9[医药卫生—肿瘤]