乙酰肝素酶反义寡核苷酸对人恶性黑素瘤裸鼠皮下移植瘤抑制作用的研究  

Inhibitory Effects of Heparinase Antisense Oligodeoxynucleotides on Growth of Human Malignant Melanoma Trans-planted Subcutaneously in Nude Mice

作　　者：于建斌[1] 张岚[2] 李琳[1] 陈燕辉[1] 陈建立[1] 张江安[1] 高冬玲[2] 陈奎生[2] 张云汉[2] 

机构地区：[1]郑州大学第一附属医院皮肤科,河南郑州450052 [2]河南省肿瘤病理重点实验室,河南郑州450052

出　　处：《中国皮肤性病学杂志》2007年第3期129-131,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的探讨乙酰肝素酶反义寡核苷酸(ASODN)对人恶性黑素瘤裸鼠皮下移植瘤的抑制作用。方法分空白组、无关序列(N-ODN)组、10μmol/LASODN组、20μmol/LASODN组和30μmol/LASODN组5组,以脂质体包埋分别转染人恶性黑素瘤细胞株A375,后接种于裸鼠皮下,观察裸鼠移植瘤体积变化,并采用原位杂交、免疫组化法检测移植瘤中乙酰肝素酶mRNA及其蛋白表达的变化。结果接种6周后,各ASODN组的裸鼠移植瘤体积均显著小于N-ODN组和对照组(P均<0.05);各ASODN组裸鼠移植瘤中乙酰肝素酶mRNA和蛋白水平均较对照组、N-ODN组显著下调(P均=0.000)。结论乙酰肝素酶ASODN对人恶性黑素瘤裸鼠皮下移植瘤具有抑制作用。Objective To investigate whether Heparinase ASODN could inhibit the growth of human malignant melanoma transplanted subcutaneously in nude mice, Methods There were five groups：control group, N-ODN group, 10 μmol/L ASODN group ,20 μmol/L ASODN group and 30 μmol/L ASODN group. The ODNs were delivered into A375 cells by OligofectamineTM reagent. The tumor volume was measured daily, Heparinase mRNA and protein in tumor tissue were detected by in situ hybridization and immunohistochemistry, respectively. Results 6 weeks after inoculation, the tumor volume in ASODN group was significantly less than that in NSODN group as well as the control group （ P 〈 0.05 ） ; the expression of heparinase mRNA and its protein was significantly lower in the ASODN group than that in N-ODN group and that in the control group （ P = 0, 000）. Conclusion Heparinase ASODN can inhibit the growth of human malignant melanoma transplanted subcutaneously in nude mice.

关 键 词：恶性黑素瘤 乙酰肝素酶 反义寡核苷酸 裸鼠 移植 

分 类 号：R739.5[医药卫生—肿瘤]